| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRBP |
| Uniprot No | Q96SI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-672 aa |
| 活性数据 | MRSIRSFAND DRHVMVKHST IYPSPEELEA VQNMVSTVEC ALKHVSDWLD ETNKGTKTEG ETEVKKDEAG ENYSKDQGGR TLCGVMRIGL VAKGLLIKDD MDLELVLMCK DKPTETLLNT VKDNLPIQIQ KLTEEKYQVE QCVNEASIII RNTKEPTLTL KVILTSPLIR DELEKKDGEN VSMKDPPDLL DRQKCLNALA SLRHAKWFQA RANGLKSCVI VLRILRDLCN RVPTWAPLKG WPLELICEKS IGTCNRPLGA GEALRRVMEC LASGILLPGG PGLHDPCERD PTDALSYMTI QQKEDITHSA QHALRLSAFG QIYKVLEMDP LPSSKPFQKY SWSVTDKEGA GSSALKRPFE DGLGDDKDPN KKMKRNLRKI LDSKAIDLMN ALMRLNQIRP GLQYKLLSQS GPVHAPVFTM SVDVDGTTYE ASGPSKKTAK LHVAVKVLQA MGYPTGFDAD IECMSSDEKS DNESKNETVS SNSSNNTGNS TTETSSTLEV RTQGPILTAS GKNPVMELNE KRRGLKYELI SETGGSHDKR FVMEVEVDGQ KFRGAGPNKK VAKASAALAA LEKLFSGPNA ANNKKKKIIP QAKGVVNTAV SAAVQAVRGR GRGTLTRGAF VGATAAPGYI APGYGTPYGY STAAPAYGLP KRMVLLPVMK FPTYPVPHYS FF |
| 分子量 | 73.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STRBP蛋白的3篇代表性文献的简要整理(信息为示例性概括，非真实文献)：
1. **文献名称**：Structural Characterization and Functional Analysis of Recombinant Human STRBP in DNA Repair
**作者**：Zhang L., et al.
**摘要**：通过大肠杆菌表达系统成功纯化重组人STRBP蛋白，证实其结合单链DNA的能力，并证明其在DNA损伤修复中促进同源重组的功能机制。
2. **文献名称**：Development of a Mammalian Cell-Expressed STRBP for Infertility Studies
**作者**：Smith J.R., et al.
**摘要**：利用哺乳动物细胞系表达具有生物活性的重组STRBP，发现其在精子发生过程中调控RNA稳定性，为男性不育症研究提供工具。
3. **文献名称**：STRBP Interaction Networks Revealed by Yeast Two-Hybrid Screening
**作者**：Wang Y., et al.
**摘要**：构建重组STRBP作为诱饵蛋白，通过酵母双杂交筛选发现其与减数分裂特异性蛋白相互作用，暗示其在生殖细胞发育中的多途径调控作用。
注：以上为模拟文献，实际研究中需通过PubMed或SciHub等平台检索真实发表的论文(关键词：recombinant human STRBP/Spermatogenesis-related protein)。
**Background of Recombinant Human STRBP (Spermatid Perinuclear RNA-Binding Protein):**
STRBP, a RNA-binding protein predominantly expressed in testis, plays a critical role in spermatogenesis and post-transcriptional gene regulation. It interacts with perinuclear structures during spermatid elongation, facilitating mRNA stabilization, transport, or translation in developing male germ cells. Structurally, STRBP contains RNA recognition motifs (RRMs) and phosphorylation sites, enabling its function in RNA metabolism.
Dysregulation of STRBP is linked to male infertility, as mutations or altered expression impair spermiogenesis, leading to abnormal sperm morphology or motility. Beyond reproduction, STRBP has emerging roles in cancer progression, where it may modulate oncogenic RNA networks.
Recombinant human STRBP, produced via *E. coli* or mammalian expression systems, is utilized to study its molecular mechanisms, RNA-binding specificity, and interactions. It aids in developing diagnostic tools for infertility and exploring therapeutic targets in reproductive disorders or cancers. Its recombinant form allows standardized in vitro assays, antibody production, and structural analyses, advancing research into germ cell biology and RNA-based regulatory pathways.
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