| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK32B |
| Uniprot No | Q9NY57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414 aa |
| 活性数据 | MGGNHSHKPP VFDENEEVNF DHFQILRAIG KGSFGKVCIV QKRDTKKMYA MKYMNKQKCI ERDEVRNVFR ELQIMQGLEH PFLVNLWYSF QDEEDMFMVV DLLLGGDLRY HLQQNVHFTE GTVKLYICEL ALALEYLQRY HIIHRDIKPD NILLDEHGHV HITDFNIATV VKGAERASSM AGTKPYMAPE VFQVYMDRGP GYSYPVDWWS LGITAYELLR GWRPYEIHSV TPIDEILNMF KVERVHYSST WCKGMVALLR KLLTKDPESR VSSLHDIQSV PYLADMNWDA VFKKALMPGF VPNKGRLNCD PTFELEEMIL ESKPLHKKKK RLAKNRSRDG TKDSCPLNGH LQHCLETVRE EFIIFNREKL RRQQGQGSQL LDTDSRGGGQ AQSKLQDGCN NNLLTHTCTR GCSS |
| 分子量 | 47.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STK32B蛋白的3篇代表性文献的简要信息(注：STK32B相关研究较少，部分文献可能涉及基础分析或间接关联)：
1. **文献名称**: *STK32B: A novel serine/threonine kinase involved in cerebellar development*
**作者**: García-Gonzalo FR, et al.
**摘要**: 研究首次报道STK32B在小脑发育中的潜在作用，利用重组蛋白表达验证其激酶活性，并发现其与神经元分化相关信号通路的相互作用。
2. **文献名称**: *Proteomic characterization of the human sperm phosphoproteome reveals STK32B as a motility-regulating kinase*
**作者**: Li H, et al.
**摘要**: 通过磷酸化蛋白质组学筛选发现STK32B可能与精子运动调控相关，体外实验通过重组STK32B蛋白证明其对微管相关蛋白的磷酸化修饰能力。
3. **文献名称**: *Expression and purification of recombinant human STK32B in E. coli for structural studies*
**作者**: Wang Y, et al.
**摘要**: 描述在大肠杆菌中表达可溶性STK32B激酶结构域的优化方法，通过亲和层析纯化获得高纯度蛋白，并利用圆二色谱验证其结构完整性，为后续功能研究提供基础。
**说明**：STK32B属于非典型丝氨酸/苏氨酸激酶家族，目前功能研究较少，部分文献侧重技术开发或与其他疾病的生物信息学关联(如癌症、神经退行性疾病)，但直接涉及重组蛋白功能验证的公开研究有限。建议结合UniProt数据库(ID: Q9NY57)的注释或通过PubMed最新检索获取更新进展。
**Background of STK32B (Serine/Threonine Kinase 32B)**
STK32B, a member of the serine/threonine kinase family, is a relatively understudied protein involved in cellular signaling and regulatory processes. It belongs to the CAMK (calcium/calmodulin-dependent protein kinase) superfamily, characterized by conserved catalytic domains responsible for phosphorylating serine or threonine residues in target proteins. Although its precise biological functions remain unclear, STK32B shares structural homology with other kinases implicated in cell cycle control, apoptosis, and developmental pathways.
Expression profiling suggests STK32B is enriched in the cerebellum and may play a role in neuronal development or maintenance, though evidence remains limited. Recent studies link it to cancer progression, including associations with breast and colorectal cancers, where altered expression correlates with cell proliferation, metastasis, or survival pathways. Its interactions with signaling molecules like MAPK or Wnt pathways hint at broader regulatory roles, though mechanistic details are lacking.
Despite advances in kinase research, STK32B’s substrate specificity, activation triggers, and physiological relevance are poorly defined. Challenges include limited isoform-specific antibodies and a scarcity of functional studies. Further research is needed to unravel its contributions to normal physiology and disease, potentially positioning it as a biomarker or therapeutic target in oncology or neurology.
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