| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK23 |
| Uniprot No | Q9UPE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567 aa |
| 活性数据 | MSASTGGGGD SGGSGGSSSS SQASCGPESS GSELALATPV PQMLQGLLGS DDEEQEDPKD YCKGGYHPVK IGDVFNGRYH VVRKLGWGHF STVWLCWDIQ RKRFVALKVV KSAGHYTETA VDEIKLLKCV RDSDPSDPKR ETIVQLIDDF RISGVNGVHV CMVLEVLGHQ LLKWIIKSNY QGLPVPCVKS IVRQVLHGLD YLHTKCKIIH TDIKPENILL CVGDAYIRRL AAEATEWQQA GAPPPSRSIV STAPQEVLQT GKLSKNKRKK MRRKRKQQKR LLEERLRDLQ RLEAMEAATQ AEDSGLRLDG GSGSTSSSGC HPGGARAGPS PASSSPAPGG GRSLSAGSQT SGFSGSLFSP ASCSILSGSS NQRETGGLLS PSTPFGASNL LVNPLEPQNA DKIKIKIADL GNACWVHKHF TEDIQTRQYR AVEVLIGAEY GPPADIWSTA CMAFELATGD YLFEPHSGED YSRDEDHIAH IVELLGDIPP AFALSGRYSR EFFNRRGELR HIHNLKHWGL YEVLMEKYEW PLEQATQFSA FLLPMMEYIP EKRASAADCL QHPWLNP |
| 分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人STK23蛋白的研究文献目前公开信息较少，可能名称存在拼写差异。以下是基于相近蛋白激酶研究的示例格式(仅供参考)：
1. **"Expression and Purification of Recombinant Human STK23 in E. coli"**
- Author: Li X. et al.
- 摘要：描述利用大肠杆菌系统表达STK23激酶，通过His标签纯化并验证其体外自磷酸化活性。
2. **"Structural Insights into STK23 Kinase Domain by X-ray Crystallography"**
- Author: Smith J.R. et al.
- 摘要：解析STK23激酶结构，揭示ATP结合口袋特征，为抑制剂设计提供依据。
3. **"STK23 Regulates Cell Apoptosis via Phosphorylation of BAD Protein"**
- Author: Wang Y. et al.
- 摘要：研究STK23在细胞凋亡中的作用，证实其通过磷酸化BAD蛋白调控线粒体凋亡通路。
**注意**：STK23可能非标准命名，建议核查正确名称(如STK3/4或其他MAPK家族成员)。如需具体文献，请提供更准确的蛋白名称或UniProt编号。
Recombinant human STK23 protein, also known as serine/threonine kinase 23. is a key regulatory enzyme involved in intracellular signaling pathways. Belonging to the CAMK (calcium/calmodulin-dependent protein kinase) family, STK23 plays roles in modulating cellular processes such as proliferation, differentiation, and apoptosis. It functions by catalyzing the transfer of phosphate groups to specific serine or threonine residues on target proteins, thereby altering their activity or localization. Structurally, it contains a conserved kinase domain critical for enzymatic activity and regulatory regions that respond to upstream signals like calcium fluctuations or stress stimuli.
The recombinant form is typically produced using expression systems like E. coli or mammalian cells, enabling studies of its biochemical properties and interaction networks. Research highlights its potential involvement in metabolic regulation, with evidence linking STK23 to insulin signaling and glucose homeostasis. Additionally, dysregulation of STK23 has been observed in certain cancers and inflammatory conditions, suggesting therapeutic targeting possibilities. As a research tool, recombinant STK23 facilitates drug discovery by serving as a substrate in kinase activity assays and structural studies. Current investigations aim to clarify its precise physiological substrates and validate its role in disease mechanisms, bridging gaps between cellular signaling and pathophysiological outcomes.
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