| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAU2 |
| Uniprot No | Q9NUL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-570 aa |
| 活性数据 | MANPKEKTAM CLVNELARFN RVQPQYKLLN ERGPAHSKMF SVQLSLGEQT WESEGSSIKK AQQAVANKAL TESTLPKPVQ KPPKSNVNNN PGSITPTVEL NGLAMKRGEP AIYRPLDPKP FPNYRANYNF RGMYNQRYHC PVPKIFYVQL TVGNNEFFGE GKTRQAARHN AAMKALQALQ NEPIPERSPQ NGESGKDMDD DKDANKSEIS LVFEIALKRN MPVSFEVIKE SGPPHMKSFV TRVSVGEFSA EGEGNSKKLS KKRAATTVLQ ELKKLPPLPV VEKPKLFFKK RPKTIVKAGP EYGQGMNPIS RLAQIQQAKK EKEPDYVLLS ERGMPRRREF VMQVKVGNEV ATGTGPNKKI AKKNAAEAML LQLGYKASTN LQDQLEKTGE NKGWSGPKPG FPEPTNNTPK GILHLSPDVY QEMEASRHKV ISGTTLGYLS PKDMNQPSSS FFSISPTSNS SATIARELLM NGTSSTAEAI GLKGSSPTPP CSPVQPSKQL EYLARIQGFQ AALSALKQFS EQGLDPIDGA MNIEKGSLEK QAKHLREKAD NNQAPPGSIA QDCKKSNSAV |
| 分子量 | 62.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人STAU2蛋白的示例参考文献(注：文献为示例性质，非真实存在)：
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1. **"Recombinant Human STAU2 Protein Expression and RNA Binding Characterization"**
*Author: Chen L., et al. (2022)*
**摘要**：研究利用大肠杆菌系统表达重组人STAU2蛋白，纯化后验证其结合双链RNA的能力，并通过电泳迁移实验(EMSA)证实其特异性结合靶标RNA序列。
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2. **"Structural Insights into STAU2 Domains and Their Role in Neuronal mRNA Localization"**
*Author: Müller R., Schmidt T. (2020)*
**摘要**：解析重组STAU2蛋白的N端结构域晶体结构，揭示其如何通过微管结合域介导神经元内RNA转运，为神经发育机制提供分子基础。
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3. **"STAU2 Knockdown Alters Synaptic Plasticity via Dysregulation of Target mRNA Translation"**
*Author: Park S., et al. (2019)*
**摘要**：通过重组STAU2蛋白功能缺失实验，发现其调控突触相关mRNA的翻译，影响海马神经元突触可塑性，表明其在学习和记忆中的潜在作用。
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**提示**：实际文献建议通过PubMed或Google Scholar以关键词“recombinant STAU2 protein”“Staufen2 structure/function”检索，关注近年分子生物学及神经科学领域研究。
Staufen homolog 2 (STAU2) is a double-stranded RNA-binding protein belonging to the Staufen family, conserved across eukaryotes. It plays critical roles in post-transcriptional gene regulation by mediating mRNA transport, stability, and translation. Structurally, STAU2 contains multiple tandem double-stranded RNA-binding domains (dsRBDs) that enable interactions with diverse RNAs and protein partners. Unlike its paralog STAU1. STAU2 exhibits enriched expression in the nervous system and is essential for neurodevelopment, including neuronal migration, dendritic arborization, and synaptic plasticity.
Functionally, STAU2 associates with RNA granules, facilitating the localized translation of mRNAs in axons and dendrites, a process vital for neural circuit formation and memory consolidation. It participates in various RNA-driven processes, such as Staufen-mediated mRNA decay (SMD) and alternative splicing. Dysregulation of STAU2 has been implicated in neurodevelopmental disorders, including autism spectrum disorders and intellectual disability, as well as in cancers where it modulates oncogenic mRNA networks.
Recombinant human STAU2 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Purified STAU2 enables in vitro analyses of RNA-protein interactions, structural characterization, and screening for therapeutic compounds targeting RNA-regulatory pathways in neurological diseases or malignancies. Its study provides insights into RNA biology and disease mechanisms linked to translational dysregulation.
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