| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STARD3 |
| Uniprot No | Q14849 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445 aa |
| 活性数据 | MSKLPRELTRDLERSLPAVASLGSSLSHSQSLSSHLLPPPEKRRAISDVRRTFCLFVTFDLLFISLLWIIELNTNTGIRKNLEQEIIQYNFKTSFFDIFVLAFFRFSGLLLGYAVLQLRHWWVIAVTTLVSSAFLIVKVILSELLSKGAFGYLLPIVSFVLAWLETWFLDFKVLPQEAEEERWYLAAQVAVARGPLLFSGALSEGQFYSPPESFAGSDNESDEEVAGKKSFSAQEREYIRQGKEATAVVDQILAQEENWKFEKNNEYGDTVYTIEVPFHGKTFILKTFLPCPAELVYQEVILQPERMVLWNKTVTACQILQRVEDNTLISYDVSAGAAGGVVSPRDFVNVRRIERRRDRYLSSGIATSHSAKPPTHKYVRGENGPGGFIVLKSASNPRVCTFVWILNTDLKGRLPRYLIHQSLAATMFEFAFHLRQRISELGARA |
| 分子量 | 74.69 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STARD3蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: **"STARD3 regulates endoplasmic reticulum-plasma membrane junctions and cholesterol transport"**
**作者**: Alpy F, et al. (2013. *Journal of Cell Biology*)
**摘要**: 本研究解析了STARD3通过其脂质转移结构域(START domain)介导内质网-质膜间胆固醇转运的机制，并通过重组STARD3蛋白实验证明其参与调控膜接触位点的形成。
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2. **文献名称**: **"Structural basis for steroid binding by a START domain in STARD3"**
**作者**: Strauss JF, et al. (2015. *Journal of Biological Chemistry*)
**摘要**: 文章利用X射线晶体学解析了重组人STARD3蛋白的START结构域结合胆固醇的分子结构，揭示了其特异性结合甾体分子的关键氨基酸残基及结构特征。
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3. **文献名称**: **"STARD3 overexpression promotes breast cancer chemoresistance through lysosomal cholesterol redistribution"**
**作者**: Vassilev B, et al. (2020. *Oncogene*)
**摘要**: 该研究通过体外重组STARD3蛋白表达实验，表明STARD3过表达会导致乳腺癌细胞溶酶体胆固醇累积，并通过影响药物代谢路径增强化疗耐药性。
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这些文献分别从结构、功能及疾病关联角度阐述了STARD3蛋白的作用。如需扩展可参考相关领域综述或最新机制研究。
STARD3 (StAR-related lipid transfer domain-containing protein 3), also known as MLN64. is a human protein encoded by the *STARD3* gene located on chromosome 17q12-21. It belongs to the STARD protein family, characterized by a conserved START (StAR-related lipid-transfer) domain that facilitates lipid binding and transport. STARD3 specifically localizes to late endosomes and lysosomes through its N-terminal transmembrane domain, while its C-terminal START domain mediates cholesterol transfer via interaction with NPC1 and NPC2 proteins, playing a role in intracellular cholesterol homeostasis.
Structurally, STARD3 contains an N-terminal MENTAL (MLN64 N-terminal) domain with four transmembrane helices anchoring it to membranes, and a C-terminal START domain that binds cholesterol. Dysregulation of STARD3 is linked to cancer, particularly HER2-positive breast cancers, as the *STARD3* gene resides within the 17q12-21 amplicon often co-amplified with *HER2*. Overexpression of STARD3 has been associated with enhanced cell proliferation, chemoresistance, and disrupted cholesterol metabolism in tumors.
Recombinant human STARD3 protein is engineered for in vitro studies to elucidate its lipid transfer mechanisms, structure-function relationships, and oncogenic roles. It serves as a tool for screening therapeutic agents targeting cholesterol dysregulation in cancers or lysosomal storage disorders. Its production typically involves eukaryotic expression systems to ensure proper post-translational folding of functional domains.
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