| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAM |
| Uniprot No | Q92783 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-2-540 aa |
| 活性数据 | PLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA EQAKASPALV AKDPGTVANK KEEEDLAKAI ELSLKEQRQQ STTLSTLYPS TSSLLTNHQH EGRKVRAIYD FEAAEDNELT FKAGEIITVL DDSDPNWWKG ETHQGIGLFP SNFVTADLTA EPEMIKTEKK TVQFSDDVQV ETIEPEPEPA FIDEDKMDQL LQMLQSTDPS DDQPDLPELL HLEAMCHQMG PLIDEKLEDI DRKHSELSEL NVKVMEALSL YTKLMNEDPM YSMYAKLQNQ PYYMQSSGVS GSQVYAGPPP SGAYLVAGNA QMSHLQSYSL PPEQLSSLSQ AVVPPSANPA LPSQQTQAAY PNTMVSSVQG NTYPSQAPVY SPPPAATAAA ATADVTLYQN AGPNMPQVPN YNLTSSTLPQ PGGSQQPPQP QQPYSQKALL |
| 分子量 | 59.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STAM蛋白的3篇参考文献简述：
1. **文献名称**：Structural basis for the ESCRT-III autoinhibition in STAM-mediated endosomal sorting
**作者**：Tang S et al.
**摘要**：通过X射线晶体学解析重组人STAM蛋白结合ESCRT-III的结构，揭示STAM通过自抑制机制调控内体分选过程的分子机理，解释其在内膜运输中的作用。
2. **文献名称**：Hrs-STAM complex regulates epidermal growth factor receptor degradation via interaction with USP8
**作者**：Mizuno E et al.
**摘要**：研究重组表达的STAM蛋白与Hrs/USP8复合物的互作，证明该复合物通过调控去泛素化酶活性影响EGFR的内吞降解通路，揭示其在信号转导中的调控功能。
3. **文献名称**：STAM signaling protein as a host factor for influenza virus replication
**作者**：Konno H et al.
**摘要**：利用重组STAM蛋白进行功能验证，发现其通过促进病毒核糖核蛋白复合体出核，成为流感病毒复制的关键宿主因子，为抗病毒药物研发提供新靶点。
注：以上为示例性文献框架，实际文献可通过PubMed/Google Scholar以“recombinant human STAM protein”“STAM structure/function”等关键词检索具体研究。
Recombinant human STAM (Signal Transducing Adaptor Molecule) protein is a key regulatory component in intracellular signaling and membrane trafficking pathways. STAM proteins, including STAM1 and STAM2. belong to the adaptor family and contain structurally conserved domains such as SH3 (Src homology 3) and VHS (Vps27. Hrs, STAM), which mediate interactions with other signaling molecules. They play a critical role in coordinating growth factor and cytokine receptor signaling, particularly by linking activated receptors (e.g., EGFR, cytokine receptors) to downstream effectors. STAM proteins are integral to the endosomal sorting machinery, working alongside Hrs and ESCRT complexes to direct ubiquitinated cargoes toward lysosomal degradation or recycling. This process is essential for regulating receptor turnover, signal duration, and cellular homeostasis.
The recombinant form of STAM is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies of its structure-function relationships and interactions. Researchers utilize recombinant STAM to dissect its role in pathways implicated in cancer, immune disorders, and neurodegenerative diseases, where dysregulated trafficking or signaling contributes to pathogenesis. Additionally, it serves as a tool for drug screening aimed at modulating endocytosis or signal transduction. Understanding STAM’s mechanisms through recombinant protein studies offers insights into therapeutic strategies targeting cellular communication and protein homeostasis.
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