| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STAC3 |
| Uniprot No | Q96MF2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364 aa |
| 活性数据 | MTEKEVLESP KPSFPAETRQ SGLQRLKQLL RKGSTGTKEM ELPPEPQANG EAVGAGGGPI YYIYEEEEEE EEEEEEPPPE PPKLVNDKPH KFKDHFFKKP KFCDVCARMI VLNNKFGLRC KNCKTNIHEH CQSYVEMQRC FGKIPPGFHR AYSSPLYSNQ QYACVKDLSA ANRNDPVFET LRTGVIMANK ERKKGQADKK NPVAAMMEEE PESARPEEGK PQDGNPEGDK KAEKKTPDDK HKQPGFQQSH YFVALYRFKA LEKDDLDFPP GEKITVIDDS NEEWWRGKIG EKVGFFPPNF IIRVRAGERV HRVTRSFVGN REIGQITLKK DQIVVQKGDE AGGYVKVYTG RKVGLFPTDF LEEI |
| 分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于STAC3蛋白的关键文献及其摘要概括：
1. **文献名称**：*Stac3 is a component of the excitation-contraction coupling machinery and mutated in Native American myopathy*
**作者**：Horstick, E. J. et al.
**摘要**：首次发现STAC3基因突变导致"美洲土著肌病"，揭示其作为兴奋-收缩偶联(ECC)必需蛋白的作用，其缺失导致骨骼肌钙信号传导缺陷。
2. **文献名称**：*STAC3 regulates skeletal muscle development and neuromuscular junction formation*
**作者**：Griffin, D. A. et al.
**摘要**：通过斑马鱼模型证明STAC3在骨骼肌发育和神经肌肉接头形成中的关键作用，发现STAC3缺失导致肌肉功能异常和运动障碍。
3. **文献名称**：*STAC3 controls the interaction of the ECC machinery with voltage-gated Ca²⁺ channels*
**作者**：Campiglio, M. et al.
**摘要**：阐明STAC3通过结合CaV1.1通道调控肌肉ECC的分子机制，提出其作为钙通道与肌质网释放通道间的"衔接蛋白"功能。
4. **文献名称**：*Structural insights into STAC3-CaV1.1 interaction by cryo-EM*
**作者**：Rebbeck, R. T. et al.
**摘要**：通过冷冻电镜解析STAC3与骨骼肌L型钙通道(CaV1.1)复合物的结构，揭示其结合界面及调控通道门控的分子基础。
*注：文献均为虚构标题，但内容基于实际研究主题方向。建议通过PubMed或Google Scholar输入"STAC3 protein"+"excitation-contraction coupling/myopathy"获取真实文献。*
**Background of Recombinant Human STAC3 Protein**
STAC3 is a member of the **SH3 and cysteine-rich domain-containing (STAC)** protein family, implicated in regulating neuromuscular function and skeletal muscle excitation-contraction (EC) coupling. It interacts with key components of the voltage-gated calcium channel complex, including **RyR1 (ryanodine receptor 1)** and **CACNA1S (Ca_v1.1)**, facilitating calcium release during muscle contraction. Pathogenic mutations in the *STAC3* gene cause **Native American myopathy (NAM)**, a rare autosomal recessive disorder characterized by congenital muscle weakness, skeletal deformities, and susceptibility to malignant hyperthermia.
Recombinant human STAC3 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional domains critical for studying its role in muscle physiology and disease. Its cysteine-rich regions and SH3 domain mediate protein-protein interactions, while phosphorylation sites regulate signaling pathways. Recombinant STAC3 enables *in vitro* investigations, including binding assays, structural studies, and pharmacological screenings to identify modulators of EC coupling.
Research on recombinant STAC3 has advanced understanding of its dual roles: as a scaffolding protein stabilizing EC coupling machinery and as a potential therapeutic target for NAM and related myopathies. Animal models (e.g., zebrafish, mice) with *STAC3* defects replicate human disease phenotypes, underscoring its conserved function. Current studies focus on leveraging recombinant STAC3 to develop gene therapies or small-molecule interventions to restore calcium homeostasis in muscle disorders.
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