| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST8SIA5 |
| Uniprot No | O15466 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-376 aa |
| 活性数据 | MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRCLELRHEILEVKVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPAFLFTTQKNTPLGTKLKYEVDTSGIYHINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDVVTVNPSIITERFHKLEKWRRPFYRVLQVYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQYLVNVSRYWLSLGVRAKRISTGLILVTAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCSCC |
| 分子量 | 70.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ST8SIA5蛋白的3篇代表性文献(根据公开研究资料整理，非真实文献，仅供示例参考)：
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1. **文献名称**: *ST8SIA5-Mediated Polysialylation in Tumor Metastasis*
**作者**: Kim H, et al.
**摘要**: 该研究揭示了重组人ST8SIA5蛋白通过催化神经细胞黏附分子(NCAM)的多唾液酸化修饰，促进肿瘤细胞的迁移和侵袭能力。实验证明，过表达ST8SIA5的癌细胞在小鼠模型中转移率显著升高。
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2. **文献名称**: *Structural Insights into Human ST8SIA5 Sialyltransferase*
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了重组人ST8SIA5蛋白的三维结构，揭示了其底物结合位点和催化机制的关键氨基酸残基，为开发靶向ST8SIA5的抑制剂提供了结构基础。
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3. **文献名称**: *ST8SIA5 Deficiency and Neurodevelopmental Disorders*
**作者**: Sato C, et al.
**摘要**: 构建了重组ST8SIA5蛋白的功能缺失模型，发现该蛋白在神经突触形成中起关键作用。基因敲除小鼠表现出学习和记忆能力缺陷，提示其与人类神经发育疾病的相关性。
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注：以上文献为模拟示例，实际文献需通过PubMed或Web of Science等数据库检索。如需真实文献，可提供具体研究主题或应用场景进一步筛选。
Recombinant human ST8SIA5 protein is a genetically engineered form of the ST8 alpha-N-acetyl-neuraminide alpha-2.8-sialyltransferase 5 enzyme, which belongs to the sialyltransferase family. This enzyme catalyzes the transfer of sialic acid residues to glycoproteins and glycolipids, specifically forming polysialic acid (polySia) chains—a post-translational modification critical for modulating cell adhesion, signaling, and neural plasticity. ST8SIA5 is predominantly expressed in brain tissues and plays roles in neurodevelopment, synaptic remodeling, and memory formation by regulating neural cell interactions through polySia-modified carriers like NCAM (Neural Cell Adhesion Molecule).
Dysregulation of ST8SIA5 has been linked to pathological conditions, including neuropsychiatric disorders and cancers. Its overexpression in certain tumors promotes metastatic potential by enhancing cell motility and immune evasion. Recombinant ST8SIA5 is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, enabling in vitro studies of its enzymatic activity, substrate specificity, and interaction with inhibitors. This recombinant tool is invaluable for exploring therapeutic strategies targeting sialylation pathways in neurodegeneration, cancer immunotherapy, and glycoengineering applications.
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