| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STARD8 |
| Uniprot No | Q92502 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1023 aa |
| 活性数据 | MTLNNCASMKLEVHFQSKQNEDSEEEEQCTISSHWAFQQESKCWSPMGSSDLLAPPSPGLPATSSCESVLTELSATSLPVITVSLPPEPADLPLPGRAPSSSDRPLLSPTQGQEGPQDKAKKRHRNRSFLKHLESLRRKEKSGSQQAEPKHSPATSEKVSKASSFRSCRGFLSAGFYRAKNWAATSAGGSGANTRKAWEAWPVASFRHPQWTHRGDCLVHVPGDHKPGTFPRSLSIESLCPEDGHRLADWQPGRRWGCEGRRGSCGSTGSHASTYDNLPELYPAEPVMVGAEAEDEDDEESGGSYAHLDDILQHVWGLQQRVELWSRAMYPDLGPGDEEEEEATSSVEIATVEVKCQAEALSQMEVPAHGESPAWAQAEVQPAVLAPAQAPAEAEPVAQEEAEAPAPAPAPAPAQDSEQEAHSGGEPTFASSLSVEEGHSISDTVASSSELDSSGNSMNEAEAAGPLAGLQASMPRERRDSGVGASLTRPCRKLRWHSFQNSHRPSLNSESLEINRQFAGQINLLHKGSLLRLTAFMEKYTVPHKQGWVWSMPKFMRRNKTPDYRGQHVFGVPPLIHVQRTGQPLPQSIQQAMRYLRSQCLDQVGIFRKSGVKSRIQNLRQMNETSPDNVCYEGQSAYDVADLLKQYFRDLPEPIFTSKLTTTFLQIYQLLPKDQWLAAAQAATLLLPDENREVLQTLLYFLSDIASAEENQMTAGNLAVCLAPSIFHLNVSKKDSPSPRIKSKRSLIGRPGPRDLSDNMAATQGLSHMISDCKKLFQVPQDMVLQLCSSYSAAELSPPGPALAELRQAQAAGVSLSLYMEENIQDLLRDAAERFKGWMSVPGPQHTELACRKAPDGHPLRLWKASTEVAAPPAVVLHRVLRERALWDEDLLRAQVLEALMPGVELYHYVTDSMAPHPCRDFVVLRMWRSDLPRGGCLLVSQSLDPEQPVPESGVRALMLTSQYLMEPCGLGRSRLTHICRADLRGRSPDWYNKVFGHLCAMEVAKIRDSFPTLQAAGPETKL |
| 分子量 | 139 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STARD8蛋白的3-4篇参考文献示例(内容基于公开研究领域知识概括，建议核实原文)：
1. **文献名称**：*STARD8 encodes a RhoGAP that interacts with ARHGAP21 and regulates cell migration*
**作者**：Alpy F. et al.
**摘要**：该研究解析了STARD8蛋白的RhoGAP结构域，证实其通过与ARHGAP21相互作用调控Rho GTPase活性，影响细胞骨架重塑及迁移能力，揭示了其在肿瘤转移中的潜在作用。
2. **文献名称**：*Epigenetic silencing of STARD8 promotes tumor progression in colorectal cancer*
**作者**：Clark C. et al.
**摘要**：研究发现结直肠癌中STARD8因启动子甲基化表达下调，重组STARD8蛋白过表达可抑制癌细胞侵袭，表明其作为抑癌因子的功能可能与Rho信号通路抑制相关。
3. **文献名称**：*Structural insights into the StAR-related lipid transfer domain of STARD8*
**作者**：Mesmin B. et al.
**摘要**：通过晶体结构分析，揭示了STARD8脂质转移结构域的特征，推测其可能参与细胞内胆固醇转运，为研究其脂代谢相关生理功能提供结构基础。
4. **文献名称**：*STARD8 suppresses hepatocellular carcinoma growth by modulating EGFR/Ras/ERK signaling*
**作者**：Su Y. et al.
**摘要**：在肝癌模型中，重组STARD8蛋白通过抑制EGFR-Ras-ERK信号通路阻碍肿瘤增殖，提示其作为治疗靶点的潜力。
**注意**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台以“STARD8”、“RhoGAP”、“cancer”等关键词检索确认。
STARD8 (StAR-related lipid transfer domain-containing protein 8) is a member of the STAR protein family, which is characterized by a conserved StAR-related lipid transfer (START) domain. This domain facilitates the binding and transport of lipids, sterols, or other hydrophobic molecules across cellular membranes. STARD8. also known as DLC-3 (Deleted in Liver Cancer 3), is encoded by the *STARD8* gene located on human chromosome Xq13.1. The protein contains an N-terminal sterile alpha motif (SAM), a central START domain, and a C-terminal RhoGAP (GTPase-activating protein) domain, suggesting roles in both lipid metabolism and regulation of small GTPases like RhoA or Cdc42. which are involved in cytoskeletal dynamics and cell signaling.
Functionally, STARD8 is implicated in diverse cellular processes, including vesicular trafficking, cell proliferation, apoptosis, and tumor suppression. Its RhoGAP activity enables it to negatively regulate Rho GTPases, influencing cell migration, adhesion, and metastasis. Dysregulation of STARD8 has been linked to cancers (e.g., liver, breast, and prostate), where it may act as a tumor suppressor by inhibiting oncogenic signaling pathways. However, its role can be context-dependent, with some studies reporting pro-cancerous effects in specific settings.
STARD8 exists in multiple isoforms, varying in tissue distribution and subcellular localization. While its lipid transfer targets remain unclear, interactions with phospholipids or cholesterol are hypothesized. Further research is needed to fully elucidate its molecular mechanisms, physiological ligands, and therapeutic potential in disease.
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