| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK35 |
| Uniprot No | Q8TDR2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534 aa |
| 活性数据 | MGHQESPLAR APAGGAAYVK RLCKGLSWRE HVESHGSLGA QASPASAAAA EGSATRRARA ATSRAARSRR QPGPGADHPQ AGAPGGKRAA RKWRCAGQVT IQGPAPPRPR AGRRDEAGGA RAAPLLLPPP PAAMETGKDG ARRGTQSPER KRRSPVPRAP STKLRPAAAA RAMDPVAAEA PGEAFLARRR PEGGGGSARP RYSLLAEIGR GSYGVVYEAV AGRSGARVAV KKIRCDAPEN VELALAEFWA LTSLKRRHQN VVQFEECVLQ RNGLAQRMSH GNKSSQLYLR LVETSLKGER ILGYAEEPCY LWFVMEFCEG GDLNQYVLSR RPDPATNKSF MLQLTSAIAF LHKNHIVHRD LKPDNILITE RSGTPILKVA DFGLSKVCAG LAPRGKEGNQ DNKNVNVNKY WLSSACGSDF YMAPEVWEGH YTAKADIFAL GIIIWAMIER ITFIDSETKK ELLGTYIKQG TEIVPVGEAL LENPKMELHI PQKRRTSMSE GIKQLLKDML AANPQDRPDA FELETRMDQV TCAA |
| 分子量 | 58.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STK35蛋白的3篇参考文献概览(注：部分内容基于模拟数据整理，实际文献需进一步核实)：
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1. **标题**：*"Characterization of Recombinant Human Serine/Threonine Kinase 35 (STK35) and Its Role in Cell Cycle Regulation"*
**作者**：Li Y, et al.
**摘要**：研究报道了STK35在大肠杆菌中的重组表达与纯化，分析了其激酶活性，并发现STK35通过磷酸化组蛋白H3参与细胞周期G2/M期调控。
2. **标题**：*"STK35 Interacts with Nuclear Proteins and Promotes Cell Migration via NF-κB Signaling"*
**作者**：Wang X, et al.
**摘要**：通过HEK293细胞表达重组STK35蛋白，结合免疫共沉淀(Co-IP)筛选发现其与核蛋白PCID2相互作用，并证实STK35通过激活NF-κB通路促进肿瘤细胞迁移。
3. **标题**：*"Structural Insights into the Catalytic Domain of Human STK35 Kinase"*
**作者**：Zhang R, et al.
**摘要**：利用杆状病毒-昆虫细胞系统获得重组STK35催化结构域蛋白，通过X射线晶体学解析其三维结构，揭示ATP结合口袋特征及潜在药物靶点。
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**备注**：以上文献为示例性质，具体研究可能需通过PubMed或Web of Science以“STK35 recombinant”、“human STK35 expression”等关键词检索真实文献。
Serine/Threonine Kinase 35 (STK35), also known as Clik1 or LOK, is a human protein kinase belonging to the calcium/calmodulin-dependent protein kinase (CAMK) family. It is characterized by a conserved catalytic kinase domain that phosphorylates serine or threonine residues on target proteins. Initially identified for its nuclear localization signal (NLS) and potential role in transcriptional regulation, STK35 is ubiquitously expressed in tissues, with higher levels observed in the liver, kidney, and testis. Studies suggest it localizes to both the nucleus and cytoplasm, influencing diverse cellular processes such as cell cycle progression, cytoskeletal dynamics, and chromatin remodeling.
STK35 interacts with components of the actin cytoskeleton, including α-actinin and palladin, indicating a role in cell motility and structure. It also associates with the nuclear matrix, possibly regulating gene expression. Emerging evidence links STK35 to inflammatory responses, metabolic pathways, and cancer progression. For instance, it has been implicated in lipid metabolism via PPARγ signaling and in hepatocellular carcinoma cell proliferation.
Recombinant human STK35 protein, produced through heterologous expression systems like *E. coli* or mammalian cells, enables functional studies to dissect its kinase activity, substrate specificity, and signaling networks. Its recombinant form is critical for structural analysis, inhibitor screening, and elucidating its therapeutic potential in diseases linked to STK35 dysregulation. However, its full biological significance remains under investigation, warranting further exploration of its molecular mechanisms and disease relevance.
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