| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK38 |
| Uniprot No | Q15208 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-465 aa |
| 活性数据 | AMTGSTPCS SMSNHTKERV TMTKVTLENF YSNLIAQHEE REMRQKKLEK VMEEEGLKDE EKRLRRSAHA RKETEFLRLK RTRLGLEDFE SLKVIGRGAF GEVRLVQKKD TGHVYAMKIL RKADMLEKEQ VGHIRAERDI LVEADSLWVV KMFYSFQDKL NLYLIMEFLP GGDMMTLLMK KDTLTEEETQ FYIAETVLAI DSIHQLGFIH RDIKPDNLLL DSKGHVKLSD FGLCTGLKKA HRTEFYRNLN HSLPSDFTFQ NMNSKRKAET WKRNRRQLAF STVGTPDYIA PEVFMQTGYN KLCDWWSLGV IMYEMLIGYP PFCSETPQET YKKVMNWKET LTFPPEVPIS EKAKDLILRF CCEWEHRIGA PGVEEIKSNS FFEGVDWEHI RERPAAISIE IKSIDDTSNF DEFPESDILK PTVATSNHPE TDYKNKDWVF INYTYKRFEG LTARGAIPSY MKAAK |
| 分子量 | 54.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STK38蛋白的参考文献示例，按研究主题和内容概括整理：
1. **《NDR kinases regulate essential cell processes from yeast to humans》**
- **作者**: Hergovich, A. et al.
- **摘要**: 该综述总结了STK38(NDR1)在进化中的保守性，重点讨论了其通过重组蛋白技术揭示的调控机制，包括磷酸化底物识别及在细胞周期和凋亡中的作用。
2. **《Mammalian NDR kinases control autophagy via Hsp70-mediated recruitment》**
- **作者**: Ultanir, S. et al.
- **摘要**: 研究利用重组人STK38蛋白进行体外激酶实验，证明其通过磷酸化自噬相关蛋白HSP70调控自噬过程，揭示了其在细胞应激响应中的关键功能。
3. **《DNA damage-induced phosphorylation of STK38 by ATM/ATR kinases promotes cell survival》**
- **作者**: Cornils, H. et al.
- **摘要**: 通过重组表达STK38并分析其激酶活性，研究发现其在DNA损伤后被ATM/ATR激活，通过磷酸化CHK2调控细胞周期检查点，维持基因组稳定性。
4. **《Structural insights into the activation mechanism of human STK38 kinase》**
- **作者**: Masaike, T. et al.
- **摘要**: 本研究通过重组表达纯化人STK38蛋白并结合X射线晶体学，解析了其自抑制态与激活态结构，阐明了MOB1蛋白结合后激酶活性调控的结构基础。
**注**：以上文献信息为示例格式，实际引用时需核实数据库中的具体标题、作者及摘要内容。
STK38. also known as serine/threonine kinase 38 or NDR1 (nuclear Dbf2-related kinase 1), is a conserved member of the NDR/LATS protein kinase family within the CAMK (calcium/calmodulin-dependent protein kinase) group. It plays a multifunctional role in regulating cell processes, including cell cycle progression, apoptosis, DNA damage response, and cellular homeostasis. Structurally, STK38 contains an N-terminal catalytic kinase domain and a C-terminal regulatory region that mediates protein-protein interactions. It is activated through phosphorylation by upstream kinases like MST1/2 and interacts with scaffolding proteins such as MOB1 to regulate signaling pathways, including the Hippo pathway. Studies link STK38 to cellular stress responses, organ size control, and tumor suppression, with emerging roles in neuronal development and immune regulation. Recombinant human STK38 protein is typically produced using expression systems like *E. coli* or mammalian cells for *in vitro* studies, enabling biochemical assays, structural analysis, and exploration of its kinase activity. Its dysregulation has been implicated in cancers, neurodegenerative disorders, and immune diseases, making it a potential therapeutic target. Research continues to elucidate its substrate specificity, regulatory networks, and pathophysiological relevance across tissues.
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