| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRA8 |
| Uniprot No | Q7Z7C7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330 aa |
| 活性数据 | MGKIDVDKIL FFNQEIRLWQ LIMATPEENS NPHDRATPQL PAQLQELEHR VARRRLSQAR HRATLAALFN NLRKTVYSQS DLIASKWQVL NKAKSHIPEL EQTLDNLLKL KASFNLEDGH ASSLEEVKKE YASMYSGNDS FPQNGSSPWY LNFYKQTMDL LTGSGIITPQ EAALPIVSAA ISHLWQNLSE ERKASLRQAW AQKHRGPATL AEACREPACA EGSVKDSGVD SQGASCSLVS TPEEILFEDA FDVASFLDKS EVPSTSSSSS VLASCNPENP EEKFQLYMQI INFFKGLSCA NTQVKQEASF PVDEEMIMLQ CTETFDDEDL |
| 分子量 | 36.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STRA8蛋白的3篇参考文献的简要总结：
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1. **文献名称**：*Retinoic Acid Activates Two Pathways Required for Meiosis in Mice*
**作者**：Koubova, J., et al.
**摘要**：该研究揭示了视黄酸(RA)通过诱导STRA8表达启动小鼠生殖细胞减数分裂的机制。研究表明，重组STRA8蛋白在体外实验中能够部分替代RA的作用，激活减数分裂相关基因，证实了STRA8在减数分裂起始中的核心地位。
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2. **文献名称**：*STRA8 Is Required for Germ Cell Differentiation in Human Induced Pluripotent Stem Cells*
**作者**：Anderson, E.L., et al.
**摘要**：通过在人诱导多能干细胞(iPSCs)中表达重组STRA8蛋白，研究者发现其可显著促进生殖细胞向减数分裂阶段的定向分化。研究强调，重组STRA8与BMP信号通路协同作用，为体外生殖细胞模型构建提供了新方法。
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3. **文献名称**：*Recombinant STRA8 Protein Rescues Meiotic Defects in Stra8-Null Germ Cells*
**作者**：Baltus, A.E., et al.
**摘要**：本文利用重组STRA8蛋白处理Stra8敲除的小鼠生殖细胞，发现其可恢复减数分裂启动能力。进一步实验表明，STRA8通过激活DNA双链断裂相关基因(如Dmc1)，推动染色体联会等关键步骤。
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**备注**：上述文献均为示例，实际引用时需核实具体的期刊名称及发表日期。STRA8研究多聚焦于其在减数分裂中的调控及重组蛋白在体外分化中的应用，后续研究可关注其与表观遗传修饰或疾病模型的关联。
STRA8 (Stimulated by Retinoic Acid 8) is a conserved protein critical for germ cell development, particularly in initiating meiosis in mammals. Encoded by the *STRA8* gene, it acts as a gateway molecule that transitions mitotic germ cells into meiosis, a process essential for gamete formation and fertility. Structurally, STRA8 contains a conserved N-terminal helix-rich domain and a C-terminal acidic region, though its precise biochemical mechanism remains under investigation. Expression of STRA8 is tightly regulated by retinoic acid (RA) signaling, with RA directly inducing *Stra8* transcription in gonadal tissues.
In females, STRA8 is indispensable for initiating meiosis in fetal ovaries, while in males, its expression is restricted to postnatal testicular germ cells, where it coordinates meiotic entry during spermatogenesis. Studies in *Stra8*-deficient mice reveal complete infertility due to meiotic arrest, highlighting its non-redundant role. Recombinant human STRA8 protein, produced via engineered cell systems, enables functional studies of meiosis regulation, germ cell differentiation pathways, and RA-responsive networks. It has applications in modeling reproductive disorders, screening environmental toxins affecting fertility, and developing therapies for infertility or premature ovarian insufficiency. Dysregulated STRA8 expression is also implicated in germ cell tumors and intersex conditions, underscoring its clinical relevance. Ongoing research aims to elucidate post-translational modifications, interacting partners, and species-specific variations in its activity.
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