| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STRADB |
| Uniprot No | Q9C0K7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418 aa |
| 活性数据 | MSLLDCFCTS RTQVESLRPE KQSETSIHQY LVDEPTLSWS RPSTRASEVL CSTNVSHYEL QVEIGRGFDN LTSVHLARHT PTGTLVTIKI TNLENCNEER LKALQKAVIL SHFFRHPNIT TYWTVFTVGS WLWVISPFMA YGSASQLLRT YFPEGMSETL IRNILFGAVR GLNYLHQNGC IHRSIKASHI LISGDGLVTL SGLSHLHSLV KHGQRHRAVY DFPQFSTSVQ PWLSPELLRQ DLHGYNVKSD IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD ISIFPQSESR MKNSQSGVDS GIGESVLVSS GTHTVNSDRL HTPSSKTFSP AFFSLVQLCL QQDPEKRPSA SSLLSHVFFK QMKEESQDSI LSLLPPAYNK PSISLPPVLP WTEPECDFPD EKDSYWEF |
| 分子量 | 47.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STRADB蛋白的示例参考文献(内容基于公开研究主题总结，非真实文献，仅供格式参考)：
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1. **Title**: "Structural basis for the activation of LKB1 by STRADβ and MO25"
**Author**: Zeqiraj, E. et al.
**Summary**: 研究解析了STRADβ与LKB1、MO25形成的三元复合物结构，揭示STRADβ通过构象变化激活LKB1激酶活性，调控能量感应通路(如AMPK信号)的分子机制。
2. **Title**: "Recombinant expression and functional characterization of human STRADβ in cell polarity regulation"
**Author**: Baas, A.F. et al.
**Summary**: 报道了重组人STRADβ蛋白的真核表达方法，并证明其与LKB1协同作用，促进上皮细胞极性的建立及肿瘤抑制功能。
3. **Title**: "STRADβ enhances AMPK signaling by facilitating LKB1-dependent phosphorylation"
**Author**: Hawley, S.A. et al.
**摘要**: 通过体外重组实验证实，STRADβ作为支架蛋白增强LKB1对AMPK的磷酸化效率，阐明了其在代谢应激响应中的关键作用。
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**说明**：以上文献名为示例，实际引用需检索PubMed等数据库(可搜索关键词：STRADB, LKB1. AMPK, recombinant expression)。真实研究多聚焦STRADB与LKB1复合物在能量代谢、细胞极性及癌症中的功能。
**Recombinant human STRADβ (STE20-related kinase adapter protein β)** is a regulatory protein involved in cellular energy sensing, polarity, and growth control. Structurally, it belongs to the STRAD family of pseudokinases, characterized by a kinase-like domain lacking catalytic activity but retaining ATP-binding capacity. STRADβ typically functions as a scaffolding partner for the tumor suppressor kinase LKB1 (STK11), forming a tripartite complex with LKB1 and STRADα or the murine-specific STRADβ isoform. This interaction is critical for LKB1 activation, enabling its phosphorylation of AMPK family kinases that regulate metabolic homeostasis, cell cycle arrest, and polarity pathways.
Unlike STRADα, STRADβ exhibits tissue-specific expression patterns, notably enriched in neural and reproductive tissues, suggesting divergent physiological roles. Dysregulation of STRADβ-LKB1 signaling is implicated in cancer progression, neurological disorders, and metabolic syndromes. Recombinant STRADβ proteins, often generated via bacterial or mammalian expression systems with affinity tags (e.g., GST, His-tags), enable mechanistic studies of LKB1 activation, ATP-binding pseudokinase functions, and disease-related mutations. Its utility extends to drug discovery targeting AMPK-related pathways and modeling STRADβ-associated pathologies, such as polyhydramnios-megalencephaly syndromes linked to STRADα/β mutations.
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