| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX4A |
| Uniprot No | Q12846 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297 aa |
| 活性数据 | MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICVSITVVLLAVIIGVTVVG |
| 分子量 | 60.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人STX4A(Syntaxin-4A)蛋白的典型研究文献示例(信息为学术示例，请核实具体文献的准确性)：
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1. **文献名称**: *"Syntaxin 4 Mediates Exocytosis of GLUT4 Storage Vesicles in Adipocytes"*
**作者**: William, H. et al. (2015)
**摘要**: 研究揭示了STX4A在脂肪细胞中调控GLUT4囊泡与质膜融合的关键作用，证实重组STX4A蛋白过表达可增强胰岛素刺激下的葡萄糖摄取，提示其潜在抗糖尿病应用。
2. **文献名称**: *"Molecular Mechanism of Syntaxin-4A in Regulating Pancreatic β-Cell Exocytosis"*
**作者**: Kowluru, A. et al. (2020)
**摘要**: 探讨STX4A与SNARE复合体互作如何促进胰岛β细胞胰岛素分泌，通过重组蛋白实验证明STX4A磷酸化修饰可影响其促分泌功能，为2型糖尿病提供新治疗靶点。
3. **文献名称**: *"Structural Analysis of STX4A-VAMP2 Complex in Membrane Fusion"*
**作者**: Chen, Y. et al. (2018)
**摘要**: 通过冷冻电镜解析重组STX4A与VAMP2的复合体结构，揭示了Syntaxin-4A在细胞内膜运输中的构象变化机制，为靶向膜融合的分子设计提供依据。
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**注意**：上述文献名称和作者为虚拟示例，实际研究中可参考以下真实期刊中的相关论文：
- *Journal of Biological Chemistry* (STX4A与代谢疾病方向)
- *Diabetes*(胰岛β细胞分泌相关)
- *Nature Cell Biology*(膜融合分子机制)
建议通过PubMed或Google Scholar以“STX4A/Syntaxin-4A recombinant protein”为关键词筛选具体文献。
**Background of Recombinant Human STX4A Protein**
Syntaxin 4A (STX4A), a member of the SNARE protein family, plays a critical role in intracellular membrane fusion events, particularly in vesicle trafficking and exocytosis. It is highly expressed in insulin-sensitive tissues, such as skeletal muscle and adipose tissue, where it regulates the translocation of glucose transporter 4 (GLUT4) vesicles to the plasma membrane—a process essential for glucose uptake and insulin signaling. Dysregulation of STX4A has been linked to metabolic disorders, including type 2 diabetes, due to its impact on insulin resistance. Structurally, STX4A contains a C-terminal transmembrane domain and a conserved SNARE motif that mediates interactions with partner proteins (e.g., SNAP-25 and VAMP2) to form the SNARE complex, driving membrane fusion.
Recombinant STX4A protein is produced using expression systems like *E. coli* or mammalian cell lines, enabling studies of its biochemical properties and interactions. Its recombinant form retains native functionality, making it valuable for *in vitro* assays, drug screening, and mechanistic research into diabetes, neurodegenerative diseases, and cancer. Recent studies also highlight its potential in therapeutic strategies aimed at restoring insulin sensitivity or manipulating vesicular transport in pathological conditions. As a research tool, recombinant STX4A provides insights into cellular trafficking dynamics and pathways underlying metabolic health and disease.
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