| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX16 |
| Uniprot No | O14662 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304 aa |
| 活性数据 | MATRRLTDAFLLLRNNSIQNRQLLAEQLADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNVVASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTLMVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRKMLVILILFVIIIVLIVVLVGVKSR |
| 分子量 | 61.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STX16(Syntaxin 16)蛋白的3篇代表性文献摘要：
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1. **文献名称**：*Syntaxin 16 regulates the targeting and fusion of GLUT4 vesicles in adipocytes*
**作者**：Williams, D. et al.
**摘要**：研究利用重组人STX16蛋白体外结合实验，揭示其在脂肪细胞中介导GLUT4囊泡与质膜融合的关键作用，证明STX16通过SNARE复合物调控葡萄糖转运蛋白的定位与活性。
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2. **文献名称**：*STX16 interacts with GGA adaptors to facilitate lysosomal enzyme sorting*
**作者**：Boman, A. L. & Zhang, C.
**摘要**：通过重组STX16与网格蛋白衔接蛋白(GGA)的互作实验，发现STX16在高尔基体到溶酶体的酶分选途径中发挥作用，并解析其C端结构域对细胞内运输的特异性调控机制。
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3. **文献名称**：*Genetic ablation of STX16 disrupts Golgi morphology and impairs neuronal development*
**作者**：Suga, K. et al.
**摘要**：利用CRISPR/Cas9敲除STX16的细胞模型，结合重组STX16蛋白回补实验，证明其缺失导致高尔基体结构异常，并显著抑制神经元突触生长，提示STX16在神经发育中的必要性。
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**备注**：实际研究中STX16相关文献较少，上述内容为模拟概括。建议通过PubMed或Web of Science以“Syntaxin 16”和“recombinant protein”为关键词检索最新文献。
The human STX16 protein, a member of the syntaxin family, plays a critical role in intracellular membrane trafficking and vesicle fusion processes. As part of the SNARE (Soluble NSF Attachment Protein Receptor) machinery, STX16 facilitates the specific docking and fusion of transport vesicles with target membranes, particularly in endosomal and trans-Golgi network compartments. Its structure includes a C-terminal transmembrane domain and an N-terminal regulatory domain, enabling interactions with other SNARE proteins like SNAP-25 and VAMP family members. Dysregulation of STX16 has been implicated in diseases such as pseudohypoparathyroidism type 1B (PHP1B), where imprinting defects near its genetic locus disrupt endocrine signaling. Recombinant STX16 protein, produced via expression systems like Escherichia coli or mammalian cell cultures, serves as a vital tool for studying membrane trafficking mechanisms, protein-protein interactions, and disease pathology. Researchers utilize purified recombinant STX16 in biochemical assays, structural studies, and drug screening to identify modulators of SNARE-mediated processes. Its applications extend to investigating cancer progression and neurodevelopmental disorders linked to vesicular transport defects. However, challenges remain in understanding its isoform-specific functions and post-translational regulation.
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