| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX5A |
| Uniprot No | Q13190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301 aa |
| 活性数据 | MSCRDRTQEFLSACKSLQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTIVELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIFVVFLA |
| 分子量 | 58.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STX5A蛋白的参考文献示例(注：以下为假设性文献，实际引用需根据真实研究调整)：
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1. **文献名称**：*Recombinant human STX5A mediates Golgi-ER retrograde transport in vitro*
**作者**：Tanaka K, et al.
**摘要**：研究通过体外实验证明，重组人STX5A蛋白与特定SNARE蛋白(如Sec22b)结合，调控高尔基体到内质网的逆向囊泡运输，揭示了其在细胞内运输中的关键作用。
2. **文献名称**：*Structural insights into the STX5A-SNAP-23-VAMP4 complex in membrane fusion*
**作者**：Guo X, Li Y
**摘要**：利用重组人STX5A蛋白进行冷冻电镜分析，解析其与SNAP-23、VAMP4形成的SNARE复合体结构，阐明膜融合过程中构象变化的分子机制。
3. **文献名称**：*High-yield production of recombinant STX5A in mammalian cells for functional studies*
**作者**：Müller R, et al.
**摘要**：开发一种基于哺乳动物细胞表达系统的重组STX5A蛋白高效生产方法，验证其生物活性，并应用于细胞转运缺陷模型的修复实验。
4. **文献名称**：*STX5A deficiency impairs collagen secretion and promotes fibrosis via recombinant protein rescue*
**作者**：Park S, et al.
**摘要**：通过外源添加重组STX5A蛋白，恢复STX5A敲除细胞中胶原蛋白的分泌能力，证实其在细胞外基质稳态和纤维化疾病中的潜在调控作用。
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以上内容为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。如需具体文章，建议使用关键词“recombinant STX5A”“human Syntaxin 5A”进一步筛选。
**Background of Recombinant Human STX5A (Syntaxin 5A) Protein**
Syntaxin 5A (STX5A) is a key member of the SNARE (Soluble *N*-ethylmaleimide-sensitive factor Attachment protein REceptor) protein family, which mediates intracellular membrane fusion events critical for vesicular trafficking. It exists in two isoforms (long and short) generated by alternative splicing, both localized to the endoplasmic reticulum (ER) and Golgi apparatus. STX5A facilitates the docking and fusion of transport vesicles by forming specific SNARE complexes with partners like Bet1. Sec22b, and the membrane-tethering protein p115 or GM130. ensuring cargo sorting between the ER, Golgi, and intermediate compartments.
Recombinant human STX5A protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional roles. It is widely used in *in vitro* assays to dissect COPII/COPI vesicle dynamics, Golgi-ER retrograde transport, and glycosylation pathways. Dysregulation of STX5A is linked to diseases, including cancer, neurodegenerative disorders, and congenital glycosylation defects. Recombinant forms enable targeted exploration of its interactions, post-translational modifications, and therapeutic potential. Its high purity and activity make it a vital tool for elucidating membrane trafficking mechanisms and developing related biomedical interventions.
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