| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STX3A |
| Uniprot No | Q13277 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-289 aa |
| 活性数据 | MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKLIIIIVLVVVLLGILALIIGLSVGLN |
| 分子量 | 57.42 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人STX3A(Syntaxin 3A)蛋白的3篇文献示例(均为虚构内容，仅作格式参考)：
---
1. **文献名称**: "Recombinant human STX3A regulates exocytosis in polarized epithelial cells"
**作者**: Zhang, L. et al.
**摘要**: 研究通过在大肠杆菌中表达并纯化重组人STX3A蛋白，证实其调控肠道上皮细胞顶端膜囊泡融合的功能。实验表明STX3A与SNAP23协同作用，影响细胞分泌及屏障完整性。
2. **文献名称**: "Structural characterization of STX3A in SNARE complex assembly"
**作者**: Müller, R. & Chen, W.
**摘要**: 利用重组STX3A蛋白解析其与VAMP8形成的SNARE复合体晶体结构，揭示其参与胞吐作用的分子机制，为靶向膜融合的药物开发提供依据。
3. **文献名称**: "STX3A dysfunction links to impaired insulin secretion in diabetes model"
**作者**: Gupta, S. et al.
**摘要**: 通过重组人STX3A蛋白体外实验，发现其在胰岛β细胞中调控胰岛素囊泡释放的作用，并证明糖尿病模型中STX3A表达下调导致分泌缺陷。
---
(注：以上文献为示例，实际研究中需通过PubMed等数据库以关键词“STX3A recombinant”、“Syntaxin 3A function”检索真实文献。)
**Background of Recombinant Human STX3A Protein**
Recombinant human STX3A (Syntaxin 3A) protein is a genetically engineered form of the STX3A protein, a member of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) family. Syntaxin 3A plays a critical role in intracellular membrane trafficking and vesicle fusion, particularly in polarized cells such as epithelial and neuronal cells. It localizes to the apical plasma membrane, where it mediates the docking and fusion of transport vesicles, essential for processes like epithelial cell polarity, neurotransmitter release, and the regulation of ion channels.
STX3A is implicated in cellular functions ranging from membrane repair to the secretion of digestive enzymes and mucins. Mutations or dysregulation of STX3A are linked to pathologies such as microvillus inclusion disease (MVID), a rare congenital enteropathy, highlighting its importance in gastrointestinal physiology. Recombinant STX3A is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) for research applications, enabling studies on its molecular interactions, structural biology, and role in disease mechanisms. It serves as a vital tool for dissecting SNARE complex dynamics, screening therapeutic agents, and exploring gene therapy approaches for STX3A-related disorders.
×
