| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUHW2 |
| Uniprot No | Q86YH2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-543 aa |
| 活性数据 | MEQSCEEEKE PEPQKNIQET KQVDDEDAEL IFVGVEHVNE DAELIFVGVT SNSKPVVSNI LNRVTPGSWS RRKKYDHLRK DTARKLQPKS HETVTSEAVT VLPASQLESR STDSPIIIEP LSKPDYRNSS PQVVPNNSSE LPSPLITFTD SLHHPVSTAL SVGGINESPR VSKQLSTFEV NSINPKRAKL RDGIIEGNSS ASFPSDTFHT MNTQQSTPSN NVHTSLSHVQ NGAPFPAAFP KDNIHFKPIN TNLDRENELA KTDILSLTSQ NKTFDPKKEN PIVLLSDFYY GQHKGEGQPE QKTHTTFKCL SCVKVLKNVK FMNHVKHHLE FEKQRNDSWE NHTTCQHCHR QFPTPFQLQC HIENVHTAQE PSTVCKICEL SFETDQVLLQ HMKDHHKPGE MPYVCQVCHY RSSVFADVET HFRTCHENTK NLLCPFCLKI FKTATPYMCH YRGHWGKSAH QCSKCRLQFL TFKEKMEHKT QCHQMFKKPK QLEGLPPETK VTIQVSLEPL QPGSVDVASI TVSTSDSEPS LPRSKSKISK KSH |
| 分子量 | 61.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SUHW2蛋白的3篇代表性文献(信息基于领域相关研究归纳，具体作者和标题为模拟示例)：
1. **《SUHW2 mediates transcriptional silencing through interaction with polycomb repressive complex 2》**
**作者**: Lee et al.
**摘要**: 研究揭示了SUHW2作为表观遗传调控因子的功能，通过重组SUHW2蛋白证实其与PRC2复合物结合，促进组蛋白H3K27me3修饰，调控神经干细胞分化中的基因沉默。
2. **《Recombinant SUHW2 exhibits SUMO ligase activity in vitro》**
**作者**: Gupta et al.
**摘要**: 报道了重组SUHW2蛋白在体外的SUMO化修饰活性，通过大肠杆菌表达系统纯化蛋白，验证其催化作用及对下游靶蛋白(如Notch信号通路相关分子)的调控机制。
3. **《Structural analysis of the SUHW2 zinc finger domain in DNA recognition》**
**作者**: Martinez et al.
**摘要**: 利用重组SUHW2蛋白的晶体结构解析，阐明了其锌指结构域与特定DNA序列的结合模式，揭示了其在基因启动子区域的靶向作用及突变对功能的影响。
**备注**：以上文献为概括性示例，实际研究中建议通过PubMed或Web of Science以“SUHW2”“recombinant SUHW2”等关键词检索最新文献。
**Background of Recombinant Human SUHW2 Protein**
The recombination human SUHW2 protein, also known as ARID4B (AT-rich interaction domain 4B), is a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. SUHW2 contains a conserved ARID domain that facilitates its binding to AT-rich regions of DNA, playing a role in transcriptional regulation and chromatin remodeling. It is implicated in epigenetic regulation by interacting with chromatin-modifying complexes, such as the Retinoblastoma (Rb) and Sin3 histone deacetylase (HDAC) complexes, to influence gene silencing, cell cycle progression, and differentiation. Studies suggest SUHW2 may act as a transcriptional repressor, contributing to tumor suppression or oncogenesis depending on cellular context. It is widely expressed in mammalian tissues and has been linked to neurodevelopmental processes and cancer progression, including breast and prostate cancers.
Recombinant SUHW2 protein is engineered for in vitro studies to elucidate its molecular mechanisms, structural interactions, and functional roles. Produced via bacterial or mammalian expression systems, it is purified for applications like DNA-binding assays, protein interaction studies, and screening for therapeutic compounds. Research on SUHW2 advances understanding of epigenetic dysregulation in diseases and may aid in developing targeted therapies or biomarkers. Its multifunctional nature underscores its significance in both basic and translational biomedical research.
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