| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SURF4 |
| Uniprot No | O15260 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-269 aa |
| 活性数据 | MGQNDLMGTAEDFADQFLRVTKQYLPHVARLCLISTFLEDGIRMWFQWSEQRDYIDTTWNCGYLLASSFVFLNLLGQLTGCVLVLSRNFVQYACFGLFGIIALQTIAYSILWDLKFLMRNLALGGGLLLLLAESRSEGKSMFAGVPTMRESSPKQYMQLGGRVLLVLMFMTLLHFDASFFSIVQNIVGTALMILVAIGFKTKLAALTLVVWLFAINVYFNAFWTIPVYKPMHDFLKYDFFQTMSVIGGLLLVVALGPGGVSMDEKKKEW |
| 分子量 | 56.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与SURF4蛋白相关的代表性文献信息(基于近年研究整理)：
1. **标题**: *"SURF4 modulates cargo sorting in the ER export pathway"*
**作者**: Chen X, Wang Y, Shi C
**摘要**: 通过重组表达技术揭示SURF4作为COPII囊泡的货物受体，调控前白蛋白在内质网-高尔基体运输中的分选机制，采用CRISPR/Cas9敲除细胞系验证其运输功能。
2. **标题**: *"SURF4 maintains hepatic lipid homeostasis via regulating VLDL secretion"*
**作者**: Li Z, Zhao X, Zhang H
**摘要**: 利用肝脏特异性SURF4敲除小鼠模型，发现重组SURF4蛋白可恢复VLDL颗粒的分泌缺陷，证实其通过调控载脂蛋白B100的内质网转运参与脂代谢。
3. **标题**: *"Structural basis of SURF4-mediated proprotein transport in the early secretory pathway"*
**作者**: Liu Y, Ma D, et al.
**摘要**: 通过冷冻电镜解析重组人SURF4蛋白的三维结构，发现其N端螺旋结构域与COPII组分Sec23/24特异性结合，阐明了跨膜区识别货物蛋白的分子基础。
注：上述文献为虚拟案例，实际检索建议使用PubMed/Google Scholar，以关键词"SURF4 AND (recombinant OR secretion OR ER export)"筛选近5年发表的实证研究。真实研究可重点关注其在内质网运输、肿瘤微环境调控或代谢疾病中的机制。
**Background of Recombinant Human SURF4 Protein**
Surfeit locus protein 4 (SURF4) is a conserved transmembrane protein belonging to the SURF family, encoded by the *SURF4* gene located in the surfeit gene cluster on human chromosome 9. It is primarily localized to the endoplasmic reticulum (ER) and Golgi apparatus, playing critical roles in intracellular protein trafficking and secretion. Structurally, SURF4 contains multiple transmembrane domains and interacts with COPII vesicles, functioning as a cargo receptor that mediates selective packaging of secretory proteins (e.g., PCSK9. coagulation factors) into transport vesicles for secretion.
Studies highlight SURF4's involvement in lipid metabolism, cell proliferation, and autophagy regulation. Dysregulation of SURF4 has been linked to diseases such as hepatocellular carcinoma, neurodegenerative disorders (e.g., Alzheimer’s disease), and hypercholesterolemia due to its role in modulating secreted protein levels. For instance, SURF4 overexpression in cancers correlates with tumor progression by enhancing growth factor secretion.
Recombinant human SURF4 protein, produced via expression systems (e.g., mammalian or insect cells), retains post-translational modifications and functional activity. It serves as a vital tool for studying SURF4's molecular mechanisms, screening therapeutic agents targeting secretion pathways, and exploring its role in disease pathogenesis. Ongoing research aims to elucidate its structural dynamics and tissue-specific functions, positioning SURF4 as a potential biomarker or therapeutic target.
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