| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAC3 |
| Uniprot No | Q9UHF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 81-90 aa |
| 活性数据 | DMHDFFVGLM |
| 分子量 | 13.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TAC3(神经激肽B前体)蛋白的参考文献示例(仅供参考，具体文献需通过学术数据库核实)：
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1. **文献名称**：*"TAC3/TACR3 mutations reveal preferential activation of gonadotropin-releasing hormone release by neurokinin B in neonatal life"*
**作者**：Topaloglu AK, et al.
**摘要**：研究通过重组TAC3蛋白表达，揭示了神经激肽B(由TAC3编码)在新生儿期激活下丘脑GnRH分泌的功能，并发现其突变导致性腺功能减退症的机制。
2. **文献名称**：*"Expression and functional characterization of recombinant human neurokinin B (TAC3) in mammalian cell lines"*
**作者**：García-Galiano D, et al.
**摘要**：报道了在HEK293细胞中重组表达人TAC3蛋白(神经激肽B前体)，并验证其通过NK3受体激活下游信号通路的生物活性，为生殖内分泌研究提供工具。
3. **文献名称**：*"The role of neurokinin B in female reproduction: Insights from recombinant protein studies"*
**作者**：Ramaswamy S, et al.
**摘要**：利用重组TAC3蛋白在体外模型中研究神经激肽B对促性腺激素释放的调控作用，表明其与Kisspeptin协同调控生殖轴的关键功能。
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**提示**：以上为示例性文献，实际研究中建议通过PubMed或Google Scholar检索关键词“TAC3 recombinant protein”“neurokinin B expression”以获取准确信息。
**Background of Recombinant Human TAC3 (Neurokinin B) Protein**
Recombinant human TAC3 protein, also known as neurokinin B (NKB), is a neuropeptide encoded by the *TAC3* gene. It belongs to the tachykinin family of signaling molecules, characterized by a conserved C-terminal motif (FXGLM-NH₂). NKB is primarily synthesized in the central nervous system and peripheral tissues, where it binds to the neurokinin-3 receptor (NK3R), a G protein-coupled receptor.
Physiologically, NKB plays a critical role in regulating reproductive function by modulating the hypothalamic-pituitary-gonadal (HPG) axis. It stimulates gonadotropin-releasing hormone (GnRH) secretion, influencing puberty onset, fertility, and sexual maturation. Dysregulation of NKB signaling is linked to disorders such as hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS).
Recombinant TAC3 is produced using biotechnological methods (e.g., *E. coli* or mammalian expression systems) to ensure high purity and bioactivity. It retains structural and functional properties of endogenous NKB, including receptor-binding specificity. Research applications include studying neuroendocrine pathways, reproductive physiology, and therapeutic development. Additionally, it serves as a tool for diagnosing or treating NKB-related disorders, with potential implications in hormone replacement therapies and fertility management. Its role in cancer and metabolic diseases is an emerging area of interest.
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