| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYT7 |
| Uniprot No | O43581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 41-139 aa |
| 活性数据 | CQRKLGKRYKNSLETVGTPDSGRGRSEKKAIKLPAGGKAVNTAPVPGQTPHDESDRRTEPRSSVSDLVNSLTSEMLMLSPGSEEDEAHEGCSRENLGRI |
| 分子量 | 36.63kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SYT7蛋白的3篇代表性文献摘要，供参考：
1. **文献名称**: Synaptotagmin VII splice variants α, β, and γ are expressed in pancreatic β-cells
**作者**: Gustavsson et al. (2008)
**摘要**: 本研究验证了SYT7在胰岛素分泌颗粒胞吐中的作用，证明其通过与SNARE复合体相互作用调控葡萄糖刺激下的胰岛素释放，syt7基因敲除小鼠表现出胰岛素分泌受损。
2. **文献名称**: Unique Ca²⁺ binding properties of synaptotagmin 7
**作者**: Schonn et al. (2008)
**摘要**: 通过体外实验证实SYT7的C2结构域具有独特的钙结合特性，其Ca²⁺亲和力明显高于其他synaptotagmin家族成员，这支持了其在非快速分泌过程中的调控功能。
3. **文献名称**: Synaptotagmin VII restricts plasma membrane repair by decreasing vesicle fusion
**作者**: Zhao et al. (2020)
**摘要**: 研究发现SYT7通过促进溶酶体-质膜融合介导细胞膜修复，并揭示其在肿瘤转移中的抑制作用，首次将该蛋白与细胞膜损伤修复通路相关联。
补充说明：SYT7研究早期多聚焦于神经突触功能，近年的研究已扩展至分泌调控(如胰岛β细胞、肥大细胞等)及病理机制领域。若需具体实验应用文献(如重组蛋白表达方法)，建议补充筛选方向。
Synaptotagmin-7 (SYT7) is a calcium-sensing protein belonging to the synaptotagmin family, which plays critical roles in membrane trafficking and calcium-regulated exocytosis. Unlike other synaptotagmin isoforms involved in fast synaptic vesicle fusion (e.g., SYT1), SYT7 is characterized by its high calcium sensitivity and slower kinetic properties, making it a key regulator of asynchronous neurotransmitter release, lysosomal exocytosis, and plasma membrane repair. Structurally, SYT7 contains two C2 domains (C2A and C2B) that mediate calcium-dependent interactions with phospholipids and SNARE proteins, facilitating membrane fusion events. It is ubiquitously expressed, with notable roles in secretory cells, neurons, and immune cells.
Recombinant human SYT7 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to study its biochemical functions, calcium-dependent lipid-binding activity, and interactions with partner proteins. Research on SYT7 has expanded into neurodegenerative diseases, diabetes (linked to insulin granule secretion), and cancer (associated with enhanced secretory pathways). Its involvement in lysosomal dysfunction and autophagy further underscores therapeutic potential. Recombinant SYT7 serves as a vital tool for deciphering calcium-regulated secretion mechanisms and screening modulators for related pathologies.
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