| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYNM |
| Uniprot No | O15061 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1241-1565 aa |
| 活性数据 | SAAGKVGDYFATEESVGTQTSVRQLQLGPKEGFSGQIQFTAPLSDKVELGVIGDSVHMEGLPGSSTSIRHISIGPQRHQTTQQIVYHGLVPQLGESGDSESTVHGEGSADVHQATHSHTSGRQTVMTEKSTFQSVVSESPQEDSAEDTSGAEMTSGVSRSFRHIRLGPTETETSEHIAIRGPVSRTFVLAGSADSPELGKLADSSRTLRHIAPGPKETSFTFQMDVSNVEAIRSRTQEAGALGVSDRGSWRDADSRNDQAVGVSFKASAGEGDQAHREQGKEQAMFDKKVQLQRMVDQRSVISDEKKVALLYLDNEEEENDGHWF |
| 分子量 | 42kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SYNM蛋白的模拟参考文献示例(注：文献信息为假设性示例，实际需通过学术数据库检索确认)：
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1. **标题**: "Recombinant human synemin: expression in E. coli and functional characterization"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了在大肠杆菌系统中高效表达重组人SYNM蛋白的方法，通过His标签纯化获得高纯度蛋白。体外实验表明，重组SYNM与α-actinin结合，并增强肌动蛋白网络的稳定性，提示其在细胞骨架动力学中的作用。
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2. **标题**: "Synemin regulates desmin filament architecture in cardiomyocytes"
**作者**: Thompson R, et al.
**摘要**: 通过重组SYNM蛋白与心肌细胞共孵育实验，发现SYNM通过C末端结构域与结蛋白(desmin)相互作用，调控中间丝组装，可能为心肌病发病机制提供新见解。
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3. **标题**: "CRISPR/Cas9-mediated SYNM knockout alters cell migration in glioblastoma models"
**作者**: Kumar S, et al.
**摘要**: 利用重组SYNM蛋白回补实验，证实SYNF缺失的胶质母细胞瘤细胞迁移能力下降，提示SYNM通过整合素信号通路影响肿瘤侵袭性。
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4. **标题**: "Structural dynamics of synemin intermediate filaments revealed by cryo-EM"
**作者**: Watanabe M, et al.
**摘要**: 通过冷冻电镜解析重组SYNM二聚体结构，揭示其杆状结构域柔性界面，为设计靶向中间丝的化合物提供结构基础。
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**提示**：实际研究中建议通过PubMed或Google Scholar以“recombinant human synemin”或“SYNM protein purification/function”为关键词检索最新文献。部分可能相关的真实作者包括P.A. Janmey、R.K.H. Liem等(需核实具体研究内容)。
Synemin, encoded by the *SYNM* gene, is a type IV intermediate filament (IF) protein expressed in muscle cells and certain non-muscle tissues. It plays structural and regulatory roles by integrating with desmin-containing IF networks, actin filaments, and microtubules to maintain cytoskeletal integrity. Synemin exists as two major isoforms (α and β) generated by alternative splicing, differing in their C-terminal domains. The α-isoform binds α-actinin, linking IFs to adhesion complexes, while the β-isoform interacts with dystrophin-associated glycoproteins, contributing to sarcolemma stability in muscle.
Recombinant human Synemin protein is typically produced in bacterial or mammalian expression systems (e.g., *E. coli* or HEK293 cells) for functional studies. Its purification often involves affinity chromatography tags. Research focuses on Synemin's roles in cell adhesion, mechanotransduction, and signaling pathways, particularly in muscle development, repair, and pathologies such as cardiomyopathy or muscular dystrophy. In cancer, Synemin overexpression correlates with metastasis, potentially regulating actin dynamics in invasive cells. Recent studies also explore its involvement in glioblastoma progression. As a scaffold protein, Synemin bridges cytoskeletal elements and signaling molecules, making it critical for understanding cellular resilience and disease mechanisms. Recombinant Synemin enables *in vitro* studies of IF network assembly and protein-protein interactions.
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