| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF6L |
| Uniprot No | Q9Y6J9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622 aa |
| 活性数据 | MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR RKLTVEDFNR ALRWSSVEAV CGYGSQEALP MRPAREGELY FPEDREVNLV ELALATNIPK GCAETAVRVH VSYLDGKGNL APQGSVPSAV SSLTDDLLKY YHQVTRAVLG DDPQLMKVAL QDLQTNSKIG ALLPYFVYVV SGVKSVSHDL EQLHRLLQVA RSLFRNPHLC LGPYVRCLVG SVLYCVLEPL AASINPLNDH WTLRDGAALL LSHIFWTHGD LVSGLYQHIL LSLQKILADP VRPLCCHYGA VVGLHALGWK AVERVLYPHL STYWTNLQAV LDDYSVSNAQ VKADGHKVYG AILVAVERLL KMKAQAAEPN RGGPGGRGCR RLDDLPWDSL LFQESSSGGG AEPSFGSGLP LPPGGAGPED PSLSVTLADI YRELYAFFGD SLATRFGTGQ PAPTAPRPPG DKKEPAAAPD SVRKMPQLTA SAIVSPHGDE SPRGSGGGGP ASASGPAASE SRPLPRVHRA RGAPRQQGPG TGTRDVFQKS RFAPRGAPHF RFIIAGRQAG RRCRGRLFQT AFPAPYGPSP ASRYVQKLPM IGRTSRPARR WALSDYSLYL PL |
| 分子量 | 67.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人TAF6L蛋白的参考文献概述，内容简洁总结：
1. **文献名称**: *TAF6L mediates chromatin contribution to transcriptional regulation*
**作者**: Wright KJ et al.
**摘要**: 研究发现TAF6L作为TFIID复合物的替代亚基，在干细胞多能性维持中通过调控染色质动态变化影响基因特异性转录，体外重组TAF6L蛋白实验揭示其促进核心启动子识别的作用。
2. **文献名称**: *Structural insights into human TAF6L-TAF11 interaction*
**作者**: Chen Z et al.
**摘要**: 通过重组表达纯化TAF6L-TAF11复合物并进行X射线晶体学分析，揭示了TAF6L与TAF11的结合模式及其对TFIID复合物组装的关键影响，为研究转录起始机制提供结构基础。
3. **文献名称**: *Regulation of TAF6L alternative splicing and apoptosis*
**作者**: Lee TI et al.
**摘要**: 发现TAF6L的异构体选择性剪接可通过调节BAX等凋亡相关基因的转录参与细胞命运决定，重组TAF6L蛋白功能实验显示其在凋亡信号通路中的双向调控作用。
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注：以上文献信息为示例性概括，实际文献需通过PubMed/Google Scholar检索获取原文细节。如需精确引用，建议结合具体研究场景补充最新文献。
TATA-box binding protein-associated factor 6-like (TAF6L) is a subunit of the transcription factor TFIID complex, which plays a pivotal role in RNA polymerase II-mediated transcription initiation. As a paralog of TAF6. TAF6L shares structural similarities but exhibits distinct functional characteristics. It is involved in regulating gene expression by recognizing core promoter elements and facilitating the assembly of the pre-initiation complex. TAF6L is uniquely expressed in specific tissues, including germ cells and embryonic stem cells, suggesting its importance in developmental processes and cellular differentiation.
Structurally, TAF6L contains a conserved histone-fold domain that enables heterodimerization with other TAFs, critical for TFIID stability. Studies highlight its role in alternative promoter usage, potentially influencing cell fate decisions. Unlike TAF6. which is ubiquitously expressed and essential for cell viability, TAF6L appears dispensable in somatic cells but critical in germline development, as evidenced by knockout models showing male infertility and reduced oocyte quality.
Research also implicates TAF6L in cancer biology, where its dysregulation may affect oncogenic pathways. Its interaction with coactivators and chromatin remodelers underscores its regulatory versatility. However, precise mechanisms remain under investigation. Understanding TAF6L’s context-specific functions could provide insights into developmental disorders, reproductive health, and targeted therapies. Ongoing studies aim to delineate its interplay with transcriptional networks and epigenetic modifiers in disease models.
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