| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF1A |
| Uniprot No | Q15573 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450 aa |
| 活性数据 | MSDFSEELKG PVTDDEEVET SVLSGAGMHF PWLQTYVETV AIGGKRRKDF AQTTSACLSF IQEALLKHQW QQAAEYMYSY FQTLEDSDSY KRQAAPEIIW KLGSEILFYH PKSNMESFNT FANRMKNIGV MNYLKISLQH ALYLLHHGML KDAKRNLSEA ETWRHGENTS SREILINLIQ AYKGLLQYYT WSEKKMELSK LDKDDYAYNA VAQDVFNHSW KTSANISALI KIPGVWDPFV KSYVEMLEFY GDRDGAQEVL TNYAYDEKFP SNPNAHIYLY NFLKRQKAPR SKLISVLKIL YQIVPSHKLM LEFHTLLRKS EKEEHRKLGL EVLFGVLDFA GCTKNITAWK YLAKYLKNIL MGNHLAWVQE EWNSRKNWWP GFHFSYFWAK SDWKEDTALA CEKAFVAGLL LGKGCRYFRY ILKQDHQILG KKIKRMKRSV KKYSIVNPRL |
| 分子量 | 52.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人TAF1A蛋白的虚构参考文献(基于研究领域常见方向，非真实文献):
1. **文献名称**: *Structural Insights into TAF1A in RNA Polymerase I Transcription*
**作者**: Zhang L, et al.
**摘要**: 通过冷冻电镜解析人源TAF1A蛋白复合体的三维结构，揭示其与RNA聚合酶I的相互作用界面，阐明其在rRNA转录起始中的构象调节机制。
2. **文献名称**: *TAF1A Deficiency Disrupts Ribosome Biogenesis in Mouse Models*
**作者**: Morimoto R, et al.
**摘要**: 构建TAF1A条件性敲除小鼠模型，发现其胚胎致死表型与rRNA加工异常直接相关，证明TAF1A在核仁压力应激响应中的关键作用。
3. **文献名称**: *Proteomic Analysis of TAF1A-Associated Chromatin Complexes*
**作者**: Gupta S, et al.
**摘要**: 采用重组TAF1A蛋白进行免疫共沉淀质谱分析，鉴定出多个新型互作蛋白(如CK2激酶)，提出TAF1A通过磷酸化修饰调节转录延伸的分子通路。
4. **文献名称**: *TAF1A Overexpression in Colorectal Cancer and Drug Targeting Potential*
**作者**: Wang X, et al.
**摘要**: 临床样本分析显示TAF1A在结直肠癌组织中显著高表达，体外实验证实抑制TAF1A可降低癌细胞的rRNA合成速率并诱导凋亡，提示其作为治疗靶点的潜力。
---
注：以上文献为模拟学术研究方向的虚构内容，实际文献需通过PubMed/Google Scholar检索关键词"TAF1A"或"TAF1A recombinant protein"验证。
Here's a concise overview of recombinant human TAF1A protein:
TAF1A (TATA-box binding protein-associated factor 1A) is a critical component of the transcription factor IID (TFIID) complex, essential for RNA polymerase II-mediated gene transcription. This 55kDa protein contains conserved domains including a histone-fold motif that facilitates heterodimer formation with other TAFs. Its primary function involves bridging interactions between core promoter elements and regulatory proteins during transcriptional initiation. Recombinant TAF1A production typically employs bacterial (E. coli) or mammalian expression systems, enabling studies of its structural and functional properties. Researchers utilize purified TAF1A to investigate TFIID assembly mechanisms, promoter recognition specificity, and transcriptional dysregulation in diseases. Recent studies suggest TAF1A's involvement in cell cycle regulation and differentiation processes, with abnormal expression patterns observed in certain cancers. The recombinant protein serves as vital material for crystallography studies, protein interaction assays, and reconstituting minimal transcription systems. Challenges in production include maintaining proper folding of its secondary structure elements critical for DNA-binding activity. Current research focuses on its potential as a therapeutic target and biomarker for transcription-related disorders.
×
