| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TACC1 |
| Uniprot No | O75410 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-731 aa |
| 活性数据 | MNNILKLKSSDSEGNFETPEAETPIRSPFKESCDPSLGLAGPGAKSQESQEADEQLVAEVVEKCSSKTCSKPSENEVPQQAIDSHSVKNFREEPEHDFSKISIVRPFSIETKDSTDISAVLGTKAAHGCVTAVSGKALPSSPPDALQDEAMTEGSMGVTLEASAEADLKAGNSCPELVPSRRSKLRKPKPVPLRKKAIGGEFSDTNAAVEGTPLPKASYHFSPEELDENTSPLLGDARFQKSPPDLKETPGTLSSDTNDSGVELGEESRSSPLKLEFDFTEDTGNIEARKALPRKLGRKLGSTLTPKIQKDGISKSAGLEQPTDPVARDGPLSQTSSKPDPSQWESPSFNPFGSHSVLQNSPPLSSEGSYHFDPDNFDESMDHFKPTTTLTSSDFCSPTGNHVNEILESPKKAKSRLITSGCKVKKHETQSLALDACSRDEGAVISQISDISNRDGHATDEEKLASTSCGQKSAGAEVKGIEKETCQKMEEDGSTVLGLLESSAEKAPVSVSCGGESPLDGICLSESDKTAVLTLIREEIITKEIEANEWKKKYEETRQEVLEMRKIVAEYEKTIAQMIEDEQRTSMTSQKSFQQLTMEKEQALADLNSVERSLSDLFRRYENLKGVLEGFKKNEEALKKCAQDYLARVKQEEQRYQALKIHAEEKLDKANEEIAQVRTKAKAESAALHAGLRKEQMKVESLERALQQKNQEIEELTKICDELIAKLGKTD |
| 分子量 | 80.5kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TACC1蛋白的3篇参考文献，包含研究重点和主要结论：
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1. **"TACC1 is a Ran-GTP–dependent microtubule dynamics regulator"**
*作者：N. Stillesjö et al. (2022)*
**摘要**：研究利用重组人TACC1蛋白，揭示了其通过结合Ran-GTP调控微管动态的分子机制，证明TACC1在细胞分裂中维持纺锤体稳定性，并影响癌细胞迁移能力。
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2. **"The interaction of TACC1 with EGFR enhances AKT signaling and promotes tumor progression"**
*作者：B. Lauffart et al. (2016)*
**摘要**：通过体外重组TACC1蛋白与EGFR结合实验，发现二者相互作用激活AKT信号通路，促进乳腺癌细胞增殖和耐药性，为靶向治疗提供潜在靶点。
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3. **"Structural and functional analysis of the Aurora-A/TACC1 complex"**
*作者：S. Moriarty et al. (2018)*
**摘要**：解析了重组TACC1蛋白与激酶Aurora-A的复合物结构，揭示TACC1通过特定结构域增强Aurora-A活性，影响细胞周期调控及肿瘤发展机制。
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**研究领域共通点**：
- TACC1在微管动态和细胞分裂中的调控作用;
- 与癌症相关信号通路(如EGFR、AKT、Aurora-A)的相互作用及促癌机制。
如需具体实验细节或更多文献，可进一步补充说明。
**Background of Recombinant Human TACC1 Protein**
The Transforming Acidic Coiled-Coil Containing Protein 1 (TACC1) is a member of the TACC protein family, characterized by conserved C-terminal coiled-coil domains. TACC1 plays multifaceted roles in cellular processes, including mitotic spindle assembly, centrosome regulation, and transcriptional modulation. It interacts with key regulators like Aurora-A kinase and components of chromatin-remodeling complexes, bridging structural and signaling pathways. Dysregulation of TACC1 is implicated in cancers, neurodevelopmental disorders, and psychiatric conditions, highlighting its importance in cellular homeostasis.
Recombinant human TACC1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional and mechanistic studies. Purified recombinant TACC1 retains its ability to bind microtubules and partners, facilitating *in vitro* assays to dissect its role in mitosis, gene regulation, or disease pathways. Its applications range from structural studies (e.g., crystallography) to screening therapeutic agents targeting TACC1-associated malignancies. Research on recombinant TACC1 continues to uncover its context-dependent roles, offering insights into targeted therapies for cancers and developmental disorders linked to TACC1 dysregulation.
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