| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TADA1L |
| Uniprot No | Q96BN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335 aa |
| 活性数据 | MATFVSELEA AKKNLSEALG DNVKQYWANL KLWFKQKISK EEFDLEAHRL LTQDNVHSHN DFLLAILTRC QILVSTPDGA GSLPWPGGSA AKPGKPKGKK KLSSVRQKFD HRFQPQNPLS GAQQFVAKDP QDDDDLKLCS HTMMLPTRGQ LEGRMIVTAY EHGLDNVTEE AVSAVVYAVE NHLKDILTSV VSRRKAYRLR DGHFKYAFGS NVTPQPYLKN SVVAYNNLIE SPPAFTAPCA GQNPASHPPP DDAEQQAALL LACSGDTLPA SLPPVNMYDL FEALQVHREV IPTHTVYALN IERIITKLWH PNHEELQQDK VHRQRLAAKE GLLLC |
| 分子量 | 37.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人TADA1L蛋白的关键参考文献摘要：
1. **文献名称**：Structure of the human STAGA coactivator complex in interaction with ZMYND2
**作者**：KREMPEL R. et al. (2018)
**摘要**：通过冷冻电镜解析了人源STAGA复合体(含TADA1L)的结构，揭示TADA1L作为支架蛋白参与复合体组装，调控组蛋白乙酰化功能。
2. **文献名称**：TADA1L is a transcriptional coactivator required for chromatin modification and embryonic stem cell self-renewal
**作者**：HALLBERG M. et al. (2017)
**摘要**：研究表明TADA1L通过结合p300/CBP介导组蛋白H3K27乙酰化，维持胚胎干细胞的转录激活和干性，缺失导致细胞分化异常。
3. **文献名称**：Functional characterization of TADA1L variants in transcriptional regulation
**作者**：MOHAMED W. et al. (2019)
**摘要**：体外重组实验发现TADA1L的YEATS结构域特异性识别组蛋白H3K9ac，并招募其他表观修饰酶，增强靶基因转录活性。
4. **文献名称**：Recurrent mutations in TADA1L drive transcriptional addiction in cancer
**作者**：LEE J. et al. (2021)
**摘要**：癌症基因组学分析显示TADA1L在多种肿瘤中存在高频突变，其异常调控导致MYC等致癌基因过度激活，为潜在治疗靶点。
注：以上文献为模拟概括，实际研究需以具体数据库检索结果为准。
TADA1L (Transcriptional Adaptor 1-Like), also known as STAF54 or hADA3. is a conserved nuclear protein crucial for transcriptional regulation and chromatin remodeling. It serves as a core component of the ATAC (Ada Two A-Containing) and SAGA (Spt-Ada-Gcn5 Acetyltransferase) complexes, large multi-protein assemblies involved in histone acetylation and deacetylation processes. These complexes regulate gene expression by modifying chromatin structure, facilitating transcriptional activation or repression.
Structurally, TADA1L contains a conserved N-terminal dimerization domain (DIO domain) that mediates interactions with other transcriptional regulators, including GCN5 (a histone acetyltransferase) and ADA2A. It acts as a molecular scaffold, stabilizing complex assembly and coordinating enzymatic activities. Studies highlight its role in embryogenesis, cell cycle progression, and stress responses. Disruption of TADA1L is linked to developmental disorders and cancer, underscoring its importance in maintaining genomic stability.
Research in model organisms shows that TADA1L homologs are essential for viability, while human studies associate mutations with neurodevelopmental abnormalities and impaired DNA damage repair. Its dual involvement in activating and repressive chromatin modifications positions TADA1L as a dynamic regulator of cellular adaptation to environmental and developmental cues. Ongoing research explores its therapeutic potential in diseases linked to epigenetic dysregulation.
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