| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R43 |
| Uniprot No | P59537 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MITFLPIIFSSLVVVTFVIGNFANGFIALVNSIESFKRQKISFADQILTALAVSRVGLLWVLLLNWYSTVLNPAFNSVEVRTTAYNIWAVINHFSNWLATTLSIFYLLKIANFSNFIFLHLKRRVKSVILVMLLGPLLFLACHLFVINMNEIVRTKEFEGNMTWKIKLKSAMYFSNMTVTMVANLVPFTLTLLSFMLLICSLCKHLKKMQLRGKGSQDPSTKVHIKALQTVISFLLLCAIYFLSIMISVWSFGSLENKPVFMFCKAIRFSYPSIHPFILIWGNKKLKQTFLSVFWQMRYWVKGEKTSSP |
| 分子量 | 61.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into the human bitter taste receptor TAS2R43 complexed with its agonist"**
- *作者：Kim et al.*
- 摘要：利用冷冻电镜技术解析了重组人TAS2R43与其天然配体(如芦荟苷)结合的复合体结构，揭示了该受体激活的关键氨基酸位点及信号传导机制。
2. **"Functional characterization of recombinant human TAS2R43 in cell-based assays"**
- *作者：Behrens & Meyerhof*
- 摘要：在HEK293细胞中表达重组TAS2R43.结合钙离子成像技术，鉴定了多种苦味化合物(如马钱子碱)的特异性激活效应，并证实其与G蛋白偶联的信号通路关联。
3. **"Genetic variation in TAS2R43 alters receptor activation and bitter taste perception"**
- *作者：Wiener et al.*
- 摘要：研究了TAS2R43基因多态性对重组蛋白功能的影响，发现特定单核苷酸多态性(SNPs)显著改变受体对人工甜味剂(如纽甜)的敏感性，解释了人群苦味感知差异。
4. **"Recombinant TAS2R43 as a potential drug target for metabolic modulation"**
- *作者：Clark & Liggett*
- 摘要：发现肠道中表达的TAS2R43可通过重组蛋白模型调节肠内分泌激素(如GLP-1)分泌，提出其作为2型糖尿病治疗靶点的可能性。
注：以上信息为虚拟生成，实际文献需通过PubMed或Web of Science等平台以关键词“TAS2R43 recombinant”或“bitter taste receptor”检索。
**Background of Human TAS2R43 Protein**
TAS2R43. a member of the taste receptor type 2 (TAS2R) family, is a G protein-coupled receptor (GPCR) primarily expressed in taste receptor cells on the tongue. It plays a critical role in bitter taste perception, a defense mechanism to detect potentially harmful compounds. TAS2R43 is activated by specific bitter substances, such as saccharin, acesulfame-K, and certain plant-derived alkaloids. Genetic variability in the *TAS2R43* gene, particularly single-nucleotide polymorphisms (SNPs), contributes to individual differences in bitter taste sensitivity, influencing dietary preferences and aversion to toxins.
Structurally, TAS2R43 features a short extracellular N-terminus and seven transmembrane domains typical of GPCRs. Unlike other sensory receptors, TAS2Rs like TAS2R43 couple with gustducin, a G-protein that initiates signaling cascades leading to neurotransmitter release and taste signal transmission. Beyond the oral cavity, TAS2R43 is found in extra-oral tissues (e.g., gastrointestinal tract, respiratory epithelium), suggesting roles in nutrient sensing, gut motility, and innate immune responses. Its expression in airways may mediate bronchoconstriction or antibacterial responses upon bitter compound detection.
Research on TAS2R43 highlights its evolutionary significance in toxin avoidance and potential therapeutic applications, such as targeting bitter receptors to modulate metabolic or immune processes.
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