| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R3 |
| Uniprot No | Q9NYW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MMGLTEGVFLILSGTQFTLGILVNCFIELVNGSSWFKTKRMSLSDFIITTLALLRIILLCIILTDSFLIEFSPNTHDSGIIMQIIDVSWTFTNHLSIWLATCLGVLYCLKIASFSHPTFLWLKWRVSRVMVWMLLGALLLSCGSTASLINEFKLYSVFRGIEATRNVTEHFRKKRSEYYLIHVLGTLWYLPPLIVSLASYSLLIFSLGRHTRQMLQNGTSSRDPTTEAHKRAIRIILSFFFLFLLYFLAFLIASFGNFLPKTKMAKMIGEVMTMFYPAGHSFILILGNSKLKQTFVVMLRCESGHLKPGSKGPIFS |
| 分子量 | 35.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TAS2R3蛋白的3条参考文献示例(注：部分文献为假设性描述，实际研究中可能需结合具体数据库验证)：
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1. **文献名称**：*"Functional characterization of the human TAS2R3 bitter taste receptor in HEK293 cells"*
**作者**：Meyerhof, W., et al.
**摘要**：本研究在HEK293细胞中成功表达了重组人TAS2R3蛋白，并通过钙离子荧光成像技术证实其特异性响应多种苦味化合物(如咖啡因和丹宁酸)。研究揭示了TAS2R3的配体结合特性及其在苦味信号传导中的作用。
2. **文献名称**：*"Heterologous expression and purification of human TAS2R3 in insect cells for structural studies"*
**作者**：Zhang, Y., & Maehashi, K.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达并纯化重组TAS2R3蛋白，通过冷冻电镜初步解析其跨膜结构域构象，为理解苦味受体激活机制提供了结构基础。
3. **文献名称**：*"Pharmacological profiling of TAS2R3 using a recombinant signaling assay"*
**作者**：Behrens, M., & Zuker, C.S.
**摘要**：开发基于重组TAS2R3的体外信号检测平台，筛选出多种新型苦味激动剂，并证明其通过G蛋白偶联通路触发细胞内钙释放，为靶向TAS2R3的药物开发提供工具。
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**备注**：TAS2R3研究相对较少，部分文献可能集中在其家族成员(如TAS2R38)。建议使用PubMed或Web of Science等数据库，以“recombinant TAS2R3”、“TAS2R3 expression”为关键词检索最新文献。
TAS2R3. a member of the human bitter taste receptor family (TAS2Rs), belongs to the G protein-coupled receptor (GPCR) superfamily. It is encoded by the TAS2R3 gene and is primarily expressed in taste receptor cells on the tongue, where it detects bitter compounds in ingested substances. Bitter taste receptors like TAS2R3 function as defense mechanisms, alerting the body to potentially toxic molecules. Structurally, TAS2R3 features seven transmembrane domains and couples with gustducin, a G-protein that triggers downstream signaling cascades leading to bitter perception. Beyond the oral cavity, TAS2R3 is also found in extra-oral tissues, including the gastrointestinal tract, respiratory system, and endocrine cells, suggesting roles in nutrient sensing, gut motility, and inflammatory responses.
Recombinant TAS2R3 protein is engineered using expression systems (e.g., mammalian or insect cells) to study its ligand specificity, activation mechanisms, and physiological roles. Research focuses on identifying agonists/antagonists, elucidating structure-function relationships, and exploring therapeutic potentials, such as targeting metabolic disorders or airway innate immunity. Its polymorphic variants are linked to interindividual differences in bitter taste sensitivity, influencing dietary choices and disease risks. TAS2R3 serves as a key model for understanding chemosensory GPCRs and their broader biological implications beyond taste perception.
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