| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCP10L |
| Uniprot No | Q8TDR4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215 aa |
| 活性数据 | MLAGQLEARD PKEGTHPEDP CPGAGAVMEK TAVAAEVLTE DCNTGEMPPL QQQIIRLHQE LGRQKSLWAD VHGKLRSHID ALREQNMELR EKLRALQLQR WKARKKSAAS PHAGQESHTL ALEPAFGKIS PLSADEETIP KYAGHKNQSA TLLGQRSSSN NSAPPKPMSL KIERISSWKT PPQENRDKNL SRRRQDRRAT PTGRPTPCAE RRGGV |
| 分子量 | 23.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCP10L蛋白的虚构示例参考文献(内容为模拟创作，非真实文献)：
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1. **文献名称**: "Expression and functional characterization of recombinant human TCP10L in mammalian cells"
**作者**: Zhang L, et al.
**摘要**: 研究通过哺乳动物表达系统成功表达重组人TCP10L蛋白，并验证其参与减数分裂过程中染色体联会的功能。实验表明TCP10L与SYCP1蛋白存在相互作用，提示其在生殖细胞发育中的潜在作用。
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2. **文献名称**: "Structural analysis of TCP10L reveals a conserved DNA-binding motif"
**作者**: Patel S, et al.
**摘要**: 通过X射线晶体学解析TCP10L蛋白结构，发现其C端含有保守的螺旋-环-螺旋(HLH)结构域，能够非特异性结合双链DNA。研究为TCP10L在基因组稳定性中的作用提供结构依据。
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3. **文献名称**: "TCP10L promotes cancer cell proliferation via PI3K/AKT signaling pathway"
**作者**: Wang Y, et al.
**摘要**: 重组人TCP10L蛋白在肺癌细胞系中过表达时，通过激活PI3K/AKT通路促进细胞增殖和迁移，提示其可能作为癌症治疗的潜在靶点。
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4. **文献名称**: "Development of a prokaryotic expression system for human TCP10L and antibody production"
**作者**: Kim H, et al.
**摘要**: 使用大肠杆菌系统高效表达可溶性重组TCP10L蛋白，并制备多克隆抗体。免疫荧光实验证实TCP10L在睾丸组织中的特异性高表达，支持其在生殖生物学中的功能。
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注：以上内容为领域逻辑推演模拟，实际文献需通过PubMed、Web of Science等数据库检索验证。
Recombinant human TCP10L (testis-specific family 10 member L) protein is a genetically engineered variant of the naturally occurring TCP10L protein, which belongs to the TCP1 (T-complex protein 1) chaperonin family. This protein is predominantly expressed in the testis, suggesting a potential role in spermatogenesis or germ cell development. Structurally, TCP10L contains conserved TCP1 domains involved in ATP binding and hydrolysis, characteristic of chaperonins that assist in protein folding and assembly. Its recombinant form is typically produced in bacterial or eukaryotic expression systems for functional studies, enabling researchers to bypass the low endogenous expression levels in native tissues.
Research on TCP10L remains limited, but emerging evidence links it to cellular processes such as cell cycle regulation and stress response. Unlike its well-studied homologs (e.g., TCP1/CCT complex), TCP10L appears to have distinct tissue-specific functions. Overexpression studies suggest interactions with cytoskeletal components and mitotic apparatus proteins, implying a potential role in meiosis during gametogenesis. Dysregulation of TCP10L has been tentatively associated with male infertility and testicular cancers, though mechanistic details are unclear. Recombinant TCP10L serves as a critical tool for antibody production, in vitro binding assays, and structural analysis to elucidate its biological significance. Current investigations focus on mapping its interaction networks and validating its proposed chaperone-like activity in protein quality control pathways.
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