| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCF7L1 |
| Uniprot No | Q9HCS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588 aa |
| 活性数据 | MPQLGGGGGG GGGGSGGGGG SSAGAAGGGD DLGANDELIP FQDEGGEEQE PSSDSASAQR DLDEVKSSLV NESENQSSSS DSEAERRPQP VRDTFQKPRD YFAEVRRPQD SAFFKGPPYP GYPFLMIPDL SSPYLSNGPL SPGGARTYLQ MKWPLLDVPS SATVKDTRSP SPAHLSNKVP VVQHPHHMHP LTPLITYSND HFSPGSPPTH LSPEIDPKTG IPRPPHPSEL SPYYPLSPGA VGQIPHPLGW LVPQQGQPMY SLPPGGFRHP YPALAMNASM SSLVSSRFSP HMVAPAHPGL PTSGIPHPAI VSPIVKQEPA PPSLSPAVSV KSPVTVKKEE EKKPHVKKPL NAFMLYMKEM RAKVVAECTL KESAAINQIL GRKWHNLSRE EQAKYYELAR KERQLHSQLY PTWSARDNYG KKKKRKREKQ LSQTQSQQQV QEAEGALASK SKKPCVQYLP PEKPCDSPAS SHGSMLDSPA TPSAALASPA APAATHSEQA QPLSLTTKPE TRAQLALHSA AFLSAKAAAS SSGQMGSQPP LLSRPLPLGS MPTALLASPP SFPATLHAHQ ALPVLQAQPL SLVTKSAH |
| 分子量 | 62.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCF7L1蛋白的参考文献概览，按研究主题分类整理：
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### 1. **《TCF7L1调控Wnt/β-catenin信号的结构与功能研究》**
**作者：** Kim et al.
**摘要：** 通过大肠杆菌系统表达重组人TCF7L1蛋白，解析其与β-catenin结合的结构域。实验表明TCF7L1通过抑制β-catenin核转运负调控Wnt通路，为癌症靶向治疗提供依据。
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### 2. **《TCF7L1在胚胎干细胞分化中的表观遗传调控》**
**作者：** Pereira et al.
**摘要：** 利用昆虫细胞表达体系制备高纯度TCF7L1.结合ChIP-seq技术揭示其通过招募组蛋白去乙酰化酶(HDACs)抑制多能性基因，驱动干细胞定向分化。
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### 3. **《结肠癌中TCF7L1与APC蛋白相互作用的分子机制》**
**作者：** Nakamura & Smith
**摘要：** 通过体外Pull-down实验证实重组TCF7L1与截短型APC蛋白异常结合，破坏Wnt信号稳态，揭示结直肠癌中TCF7L1的促癌突变效应。
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### 4. **《基于CRISPR筛选的TCF7L1转录抑制网络研究》**
**作者：** González-Sánchez et al.
**摘要：** 结合重组TCF7L1的DNA结合实验与CRISPR功能筛选，系统性鉴定其下游靶基因，提出TCF7L1通过调控EMT相关因子促进肿瘤转移的模型。
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**提示：** 以上为虚拟文献示例，实际研究中建议通过PubMed或Web of Science以“TCF7L1 recombinant protein”、“TCF7L1 purification”及“Wnt signaling”等关键词检索最新论文，优先选择近五年内发表于《Nature Cell Biology》《Cell Reports》等期刊的机制研究。
TCF7L1 (T-cell factor 7-like 1), also known as TCF3. is a member of the TCF/LEF (lymphoid enhancer-binding factor) family of transcription factors that play pivotal roles in the Wnt/β-catenin signaling pathway. This evolutionarily conserved pathway regulates critical biological processes, including embryonic development, stem cell maintenance, and tissue homeostasis. TCF7L1 acts as a context-dependent mediator of Wnt signaling by binding to DNA via its high-mobility group (HMG) domain and interacting with β-catenin to activate or repress target gene expression. Unlike other TCF family members (e.g., TCF7L2), TCF7L1 often functions as a transcriptional repressor in the absence of Wnt signaling, suppressing Wnt-responsive genes through associations with co-repressors like Groucho/TLE proteins.
Research has highlighted TCF7L1's dual role in development and disease. During embryogenesis, it is essential for axis formation and neural differentiation. In cancer, TCF7L1 exhibits both tumor-suppressive and oncogenic activities depending on tissue type and signaling context. For instance, it inhibits epithelial-mesenchymal transition (EMT) in certain cancers but promotes tumor aggressiveness in others by modulating Wnt-driven oncogenic programs. Recombinant human TCF7L1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables mechanistic studies of its DNA-binding properties, interactions with β-catenin, and regulatory effects on gene networks. Its applications span structural analysis, in vitro binding assays, and functional screening for therapeutic agents targeting Wnt-related pathologies, including cancer and metabolic disorders.
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