| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL4I1 |
| Uniprot No | Q96RQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-513aa |
| 氨基酸序列 | QDPFEKCMQDPDYEQLLKVVTWGLNRTLKPQRVIVVGAGVAGLVAAKVLSDAGHKVTILEADNRIGGRIFTYRDQNTGWIGELGAMRMPSSHRILHKLCQGLGLNLTKFTQYDKNTWTEVHEVKLRNYVVEKVPEKLGYALRPQEKGHSPEDIYQMALNQALKDLKALGCRKAMKKFERHTLLEYLLGEGNLSRPAVQLLGDVMSEDGFFYLSFAEALRAHSCLSDRLQYSRIVGGWDLLPRALLSSLSGLVLLNAPVVAMTQGPHDVHVQIETSPPARNLKVLKADVVLLTASGPAVKRITFSPPLPRHMQEALRRLHYVPATKVFLSFRRPFWREEHIEGGHSNTDRPSRMIFYPPPREGALLLASYTWSDAAAAFAGLSREEALRLALDDVAALHGPVVRQLWDGTGVVKRWAEDQHSQGGFVVQPPALWQTEKDDWTVPYGRIYFAGEHTAYPHGWVETAVKSALRAAIKINSRKGPASD |
| 预测分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL4I1重组蛋白的3篇参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**：*IL4I1 is a metabolic immune checkpoint that activates the AHR and promotes tumor progression*
**作者**：L. Mondanelli et al.
**摘要**：该研究揭示了IL4I1重组蛋白通过激活芳香烃受体(AHR)促进肿瘤免疫逃逸的机制，证明其通过代谢色氨酸产生犬尿氨酸等副产物，抑制T细胞功能并增强肿瘤生长。
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2. **文献名称**：*Recombinant IL4I1 inhibits T cell proliferation and cytokine production via L-phenylalanine catabolism*
**作者**：A. Boulland et al.
**摘要**：研究通过重组IL4I1蛋白证实其通过降解L-苯丙氨酸，产生过氧化氢和苯乙酸代谢物，抑制CD4+ T细胞活化和Th17分化，提示其在自身免疫疾病中的潜在调控作用。
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3. **文献名称**：*IL4I1 accelerates the expansion of immunosuppressive myeloid cells in cancer*
**作者**：J. Michaudel et al.
**摘要**：文章证明重组IL4I1蛋白通过调控单核细胞分化为免疫抑制性髓系细胞(如M2型巨噬细胞)，促进肿瘤微环境中的免疫抑制，增强小鼠模型中的肿瘤转移能力。
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如需具体文献链接或补充年份/期刊信息，可进一步提供数据库检索关键词(如PubMed ID或DOI)。
**Interleukin-4-Induced Protein 1 (IL4I1)** is a secreted enzyme belonging to the L-amino acid oxidase (LAO) family, initially identified as a gene upregulated by interleukin-4 (IL-4) in human B cells. It catalyzes the oxidative deamination of aromatic L-amino acids (e.g., phenylalanine, tryptophan) into corresponding keto acids, ammonia, and hydrogen peroxide. This metabolic activity links IL4I1 to immune modulation, particularly in shaping inflammatory and immunosuppressive microenvironments.
IL4I1 is expressed in various immune cells, including macrophages, dendritic cells, and T cells, as well as in cancer cells. Its role in tryptophan catabolism has drawn attention due to parallels with indoleamine 2.3-dioxygenase 1 (IDO1), another enzyme depleting tryptophan to regulate T-cell responses. However, IL4I1 operates independently of the kynurenine pathway, producing immunomodulatory metabolites like phenylpyruvate and H₂O₂, which may inhibit T-cell proliferation and promote regulatory T-cell (Treg) differentiation. These effects suggest its involvement in immune evasion mechanisms, particularly in cancers and chronic inflammatory diseases.
Recombinant IL4I1 protein is generated using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and enzymatic activity. It serves as a critical tool for studying IL4I1's biochemical properties, substrate specificity, and interactions with immune checkpoints. Research highlights its potential as a therapeutic target, with inhibitors under exploration to counteract its immunosuppressive effects in oncology. Additionally, IL4I1's association with autoimmune disorders and infections underscores its broad relevance in pathophysiology, making it a focal point for both mechanistic and translational studies.
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