| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THAP2 |
| Uniprot No | Q9H0W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228 aa |
| 活性数据 | MPTNCAAAGC ATTYNKHINI SFHRFPLDPK RRKEWVRLVR RKNFVPGKHT FLCSKHFEAS CFDLTGQTRR LKMDAVPTIF DFCTHIKSMK LKSRNLLKKN NSCSPAGPSN LKSNISSQQV LLEHSYAFRN PMEAKKRIIK LEKEIASLRR KMKTCLQKER RATRRWIKAT CLVKNLEANS VLPKGTSEHM LPTALSSLPL EDFKILEQDQ QDKTLLSLNL KQTKSTFI |
| 分子量 | 26.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THAP2蛋白的3篇参考文献示例(注：部分内容为模拟概括，实际文献需结合数据库查询)：
1. **文献名称**: *THAP2 regulates apoptosis through transcriptional control of pro-apoptotic genes*
**作者**: Zhang, Y., et al.
**摘要**: 研究揭示了THAP2蛋白通过结合靶基因启动子区域的特定序列，调控凋亡相关基因(如BAX、CASP3)的表达，并在癌细胞中抑制肿瘤生长。重组THAP2蛋白的体外实验表明其直接参与染色质重塑复合物互作。
2. **文献名称**: *Structural and functional analysis of the THAP2 zinc finger domain*
**作者**: Lee, S.H., & Kim, J.H.
**摘要**: 通过X射线晶体学解析重组人THAP2蛋白的锌指结构域，揭示其与DNA结合的特异性模式，并证明突变关键氨基酸残基会破坏其转录抑制功能，为THAP2在基因沉默中的作用提供结构基础。
3. **文献名称**: *THAP2 as a novel biomarker in hepatocellular carcinoma*
**作者**: Wang, X., et al.
**摘要**: 临床研究发现，肝癌组织中THAP2蛋白表达水平显著下调，重组THAP2过表达可抑制肝癌细胞增殖和迁移。机制研究表明THAP2通过调控Wnt/β-catenin通路发挥抑癌作用。
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**注意**：上述文献为示例性概括，实际研究中THAP2相关文献可能较少(需确认基因命名准确性)。建议通过PubMed或Web of Science以“THAP2 protein”、“recombinant THAP2”为关键词检索最新成果，并关注其与转录调控、癌症或发育疾病的相关研究。
Thapsia argentea Activating Protein 2 (THAP2), a member of the THAP domain-containing protein family, is a zinc finger transcription factor characterized by its conserved N-terminal THAP domain. This ~90-amino acid motif binds DNA through a C2CH zinc-coordinating structure, enabling sequence-specific regulation of target genes. THAP proteins are implicated in diverse cellular processes, including cell cycle control, apoptosis, and chromatin modification. While THAP2’s precise biological roles remain less characterized than other family members (e.g., THAP1), studies suggest its involvement in transcriptional repression and epigenetic regulation.
Recombinant human THAP2 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) followed by affinity purification. Its recombinant form facilitates in vitro studies to decipher DNA-binding properties, protein interaction networks (e.g., histone-modifying enzymes), and functional roles in developmental or disease contexts, such as cancer or neurological disorders. As aberrant THAP protein activity is linked to pathologies, recombinant THAP2 serves as a critical tool for structural analyses, drug screening, and mechanistic studies aiming to unravel its contributions to gene regulatory networks.
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