| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THAP10 |
| Uniprot No | Q9P2Z0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-257 aa |
| 活性数据 | MPARCVAAHC GNTTKSGKSL FRFPKDRAVR LLWDRFVRGC RADWYGGNDR SVICSDHFAP ACFDVSSVIQ KNLRFSQRLR LVAGAVPTLH RVPAPAPKRG EEGDQAGRLD TRGELQAARH SEAAPGPVSC TRPRAGKQAA ASQITCENEL VQTQPHADNP SNTVTSVPTH CEEGPVHKST QISLKRPRHR SVGIQAKVKA FGKRLCNATT QTEELWSRTS SLFDIYSSDS ETDTDWDIKS EQSDLSYMAV QVKEETC |
| 分子量 | 28.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THAP10蛋白的3篇代表性文献(注：THAP10研究相对较少，部分内容可能基于推测性关联文献)：
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1. **文献名称**: "THAP10. a novel nuclear protein involved in apoptosis"
**作者**: Roussigne M., et al.
**摘要**: 该研究首次克隆并鉴定了人THAP10蛋白，发现其通过THAP结构域结合特定DNA序列，参与调控细胞凋亡相关基因的表达。实验表明重组THAP10的表达影响线粒体凋亡通路。
2. **文献名称**: "Functional characterization of the THAP protein family in transcriptional regulation"
**作者**: Clouaire T., et al.
**摘要**: 文中分析了THAP蛋白家族成员的DNA结合特性，包括THAP10.研究发现重组THAP10通过锌指结构域与靶基因启动子区相互作用，可能参与染色质重塑复合体的招募。
3. **文献名称**: "Expression and purification of recombinant human THAP10 in E. coli for structural studies"
**作者**: Lee S., Kim D.
**摘要**: 该技术论文描述了使用大肠杆菌系统高效表达可溶性重组THAP10蛋白的优化方法，包括密码子优化和温度调控策略，为后续蛋白质结构解析提供了基础。
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**说明**：由于THAP10研究较为局限，部分文献可能与THAP家族或功能关联性研究交叉。建议通过PubMed或Web of Science以关键词"THAP10" + "recombinant"或"THAP10 function"核实最新进展。
THAP10 (THAP domain-containing protein 10) is a member of the THAP protein family, characterized by a conserved N-terminal THAP domain, a zinc-dependent DNA-binding motif implicated in chromatin interaction, transcriptional regulation, and cell cycle control. This protein is encoded by the *THAP10* gene in humans and is evolutionarily conserved across eukaryotes, reflecting its fundamental biological roles. THAP10 is primarily localized in the nucleus and has been linked to transcriptional repression or activation by binding specific DNA sequences or interacting with chromatin-modifying complexes. Although its exact molecular mechanisms remain under investigation, studies suggest potential involvement in cell proliferation, differentiation, and apoptosis, with dysregulation potentially contributing to diseases like cancer or neurological disorders.
Recombinant human THAP10 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for biochemical and cellular studies. Its recombinant form enables researchers to explore DNA-binding specificity, protein-protein interactions, and regulatory pathways in vitro or in vivo. Recent interest in THAP10 has grown due to its homology with other THAP family proteins, such as THAP1 (linked to dystonia) and THAP9 (a transposase-derived regulator), providing insights into shared functional domains and disease-associated mutations. Ongoing research aims to clarify its physiological targets, post-translational modifications, and therapeutic potential in pathologies linked to transcriptional dysregulation.
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