| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THEX1 |
| Uniprot No | Q8IV48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-349 aa |
| 活性数据 | MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASDFSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK |
| 分子量 | 66.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THEX1蛋白的模拟参考文献示例(实际文献需通过学术数据库核实)：
---
1. **文献名称**：*"Cloning and Functional Characterization of Recombinant Human THEX1 as a 3'-5' Exoribonuclease"*
**作者**：Li, X., et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人THEX1蛋白，证实其具有3'-5'外切核糖核酸酶活性，并参与RNA降解途径的调控。
2. **文献名称**：*"Structural Insights into THEX1 Protein: Implications for Substrate Recognition"*
**作者**：Wang, Y., & Zhang, H.
**摘要**：通过X射线晶体学解析了THEX1蛋白的三维结构，揭示了其活性位点的关键氨基酸残基，为设计针对RNA代谢疾病的抑制剂提供了结构基础。
3. **文献名称**：*"THEX1 Deficiency Disrupts Neuronal RNA Homeostasis in Neurodegenerative Models"*
**作者**：Chen, J., et al.
**摘要**：利用重组THEX1蛋白进行功能回补实验，发现其在神经细胞中通过清除异常RNA缓解阿尔茨海默病模型中的病理表型。
4. **文献名称**：*"Optimization of Recombinant THEX1 Protein Expression in Mammalian Cells for High-Throughput Screening"*
**作者**：Kim, S., et al.
**摘要**：优化了哺乳动物细胞系统中THEX1蛋白的分泌表达条件，显著提高了蛋白产量和酶活稳定性，适用于高通量药物筛选平台。
---
⚠️ **注意**：以上文献为示例性内容，并非真实存在的论文。检索真实文献请使用**PubMed**、**Web of Science**或**Google Scholar**等平台，关键词可尝试“THEX1 protein recombinant”“THEX1 exoribonuclease”等。
THEX1 (Thioesterase XYZ homolog 1) is a recombinant human protein belonging to the thioesterase enzyme family, which plays critical roles in lipid metabolism, protein modification, and cellular signaling. Originally identified through homology with bacterial thioesterases, THEX1 is characterized by its conserved α/β-hydrolase fold and catalytic residues that mediate hydrolysis of thioester bonds in acyl-CoA substrates. This enzymatic activity links THEX1 to fatty acid biosynthesis, energy homeostasis, and detoxification processes.
Recombinant THEX1 is produced via genetic engineering in heterologous systems like *E. coli* or mammalian cell cultures, enabling high-purity yields for research and therapeutic applications. Its production often involves codon optimization, fusion tags (e.g., His-tags), and chromatographic purification to enhance stability and functionality. Studies suggest THEX1 may regulate lipid droplet dynamics and interact with metabolic pathways disrupted in obesity, diabetes, and neurodegenerative disorders. Emerging evidence also implicates THEX1 in cancer cell survival, where its overexpression correlates with altered lipid metabolism in tumors.
As a research tool, recombinant THEX1 facilitates enzymatic assays, structural studies (e.g., X-ray crystallography), and inhibitor screening for drug development. Its potential therapeutic applications include targeting metabolic syndromes or cancers reliant on lipid reprogramming. However, in vivo roles and regulatory mechanisms remain under investigation, underscoring THEX1's significance in bridging basic biochemistry with translational medicine.
×
