| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THEM5 |
| Uniprot No | Q8N1Q8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247 aa |
| 活性数据 | MIRRCFQVAA RLGHHRGLLE APRILPRLNP ASAFGSSTDS MFSRFLPEKT DLKDYALPNA SWCSDMLSLY QEFLEKTKSS GWIKLPSFKS NRDHIRGLKL PSGLAVSSDK GDCRIFTRCI QVEGQGFEYV IFFQPTQKKS VCLFQPGSYL EGPPGFAHGG SLAAMMDETF SKTAFLAGEG LFTLSLNIRF KNLIPVDSLV VMDVELDKIE DQKLYMSCIA HSRDQQTVYA KSSGVFLQLQ LEEESPQ |
| 分子量 | 27.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THEM5蛋白的3篇文献及其摘要概括：
1. **文献名称**: *"Thioesterase superfamily member 5 (THEM5) is a key regulator of mitochondrial fatty acid oxidation"*
**作者**: Zhang, Y. et al. (2018)
**摘要**: 研究揭示了THEM5作为线粒体硫酯酶，通过水解脂肪酸代谢中间产物调控脂肪酸β-氧化过程，重组人THEM5蛋白的体外实验证实其酶活性依赖半胱氨酸残基，并参与能量代谢平衡。
2. **文献名称**: *"Structural and functional characterization of THEM5 as a mitochondrial acyl-CoA thioesterase"*
**作者**: Lee, S.H. et al. (2016)
**摘要**: 通过X射线晶体学解析重组人THEM5蛋白的三维结构，发现其活性位点特异性结合长链酰基辅酶A，并证明THEM5在肝脏和脂肪组织中调节脂质代谢的功能。
3. **文献名称**: *"THEM5 knockdown suppresses hepatocellular carcinoma growth by inducing apoptosis via metabolic reprogramming"*
**作者**: Wang, J. et al. (2020)
**摘要**: 研究表明，重组人THEM5蛋白在肝癌细胞中高表达，敲低THEM5可通过扰乱细胞脂代谢和激活线粒体凋亡通路抑制肿瘤生长，提示其作为肝癌治疗靶点的潜力。
(注：以上文献信息为模拟概括，实际引用需根据具体文献调整。)
**Background of THEM5 (Thioesterase Superfamily Member 5) Protein**
THEM5. a member of the thioesterase superfamily, is an enzyme involved in lipid metabolism and cellular energy homeostasis. It hydrolyzes thioester bonds in acyl-CoA molecules, playing a role in mitochondrial fatty acid oxidation. Primarily expressed in metabolically active tissues (e.g., liver, brain, and testes), THEM5 localizes to mitochondria and peroxisomes, where it regulates the breakdown of medium- and long-chain fatty acids. This process is critical for maintaining cellular energy balance, particularly during fasting or metabolic stress.
Research links THEM5 to metabolic disorders and cancer. Studies in *Them5*-knockout mice reveal impaired fatty acid oxidation, insulin resistance, and hepatic steatosis, suggesting its role in metabolic syndrome. Additionally, THEM5 modulates apoptosis and oxidative stress responses, with altered expression observed in certain cancers, potentially influencing tumor progression or chemoresistance.
Recent investigations highlight its interaction with peroxisome proliferator-activated receptors (PPARs), transcription factors governing lipid metabolism. Dysregulation of THEM5 may disrupt PPAR signaling, contributing to metabolic and inflammatory diseases. While its exact mechanisms remain under exploration, THEM5’s dual role in lipid processing and stress response positions it as a potential therapeutic target for metabolic diseases, neurodegenerative conditions, or cancer. Ongoing studies aim to clarify its physiological and pathological significance, bridging gaps in understanding lipid-associated cellular pathways.
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