| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIRT4 |
| Uniprot No | Q9Y6E7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRF ITLSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVRSAP IRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWLVTQNVDALHT KAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLA PDGDVFLSEEQVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRV KEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKL NSRCGELLPLIDPC |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIRT4重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic Beta Cells*
**作者**：Anderson, K.A., Huynh, F.K., Fisher-Wellman, K., et al.
**摘要**：该研究通过重组SIRT4蛋白的体外实验，揭示了其作为线粒体ADP-核糖基转移酶的功能，能够抑制谷氨酸脱氢酶(GDH)活性，从而减少胰岛素分泌。这为SIRT4在代谢调节和糖尿病中的潜在作用提供了机制解释。
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2. **文献名称**：*SIRT4 Has Tumor-Suppressive Activity and Regulates the Cellular Metabolic Response to DNA Damage by Inhibiting Mitochondrial Glutamine Metabolism*
**作者**：Ho, L., Titus, A.S., Banerjee, K.K., et al.
**摘要**：本研究利用重组SIRT4蛋白，证明其通过抑制谷氨酰胺代谢和降低细胞抗氧化能力，抑制肺癌细胞增殖。同时发现SIRT4在DNA损伤后通过调控线粒体代谢途径发挥肿瘤抑制作用。
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3. **文献名称**：*SIRT4 Represses Peroxisome Proliferator-Activated Receptor Alpha Activity to Suppress Hepatic Fatty Acid Oxidation*
**作者**：Laurent, G., de Boer, V.C.J., Finley, L.W.S., et al.
**摘要**：通过重组SIRT4蛋白的功能分析，研究发现SIRT4通过去乙酰化修饰抑制PPARα转录活性，从而下调肝脏脂肪酸氧化相关基因表达，揭示了其在脂代谢平衡中的新机制。
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**备注**：上述文献均为SIRT4功能研究的经典工作，涉及代谢调控、肿瘤抑制及酶活性机制。如需具体发表年份或期刊，可进一步通过作者和标题在PubMed/Google Scholar检索全文。
SIRT4. a member of the sirtuin family of NAD+-dependent deacylases, is a mitochondrial enzyme implicated in metabolic regulation and aging. Initially identified for its role in modulating insulin secretion in pancreatic β-cells, SIRT4 has since been recognized as a multifaceted regulator of mitochondrial metabolism. Unlike other sirtuins (e.g., SIRT1 or SIRT3) that primarily exhibit deacetylase activity, SIRT4 predominantly functions as an ADP-ribosyltransferase and lipoamidase, targeting key enzymes in metabolic pathways such as glutamate dehydrogenase (GDH), pyruvate dehydrogenase complex (PDH), and fatty acid oxidation enzymes. These post-translational modifications enable SIRT4 to suppress catabolic processes under nutrient-rich conditions, thereby fine-tuning energy homeostasis and cellular stress responses.
Recombinant SIRT4 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional SIRT4 for *in vitro* studies. This protein typically retains enzymatic activity and structural integrity, allowing researchers to investigate its substrate specificity, kinetic properties, and interactions with metabolic partners. The development of recombinant SIRT4 has been pivotal in elucidating its role in diseases, including cancer, where it acts as a tumor suppressor by repressing glutamine metabolism, and metabolic disorders like diabetes, where its dysregulation correlates with mitochondrial dysfunction.
Current research leverages recombinant SIRT4 to screen small-molecule modulators for therapeutic applications, aiming to exploit its enzymatic activity to correct metabolic imbalances. Its emerging association with longevity pathways and age-related diseases further underscores its biomedical relevance. However, challenges persist in fully characterizing its diverse enzymatic activities and tissue-specific roles, necessitating advanced recombinant protein tools to bridge mechanistic gaps. Overall, SIRT4 recombinant protein serves as a critical resource for decoding mitochondrial signaling networks and developing targeted metabolic therapies.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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