| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIRT4 |
| Uniprot No | Q9Y6E7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314aa |
| 氨基酸序列 | MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRF ITLSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVRSAP IRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWLVTQNVDALHT KAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLA PDGDVFLSEEQVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRV KEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKL NSRCGELLPLIDPC |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIRT4重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic Beta Cells*
**作者**：Anderson, K.A., Huynh, F.K., Fisher-Wellman, K., et al.
**摘要**：该研究通过重组SIRT4蛋白的体外实验，揭示了其作为线粒体ADP-核糖基转移酶的功能，能够抑制谷氨酸脱氢酶(GDH)活性，从而减少胰岛素分泌。这为SIRT4在代谢调节和糖尿病中的潜在作用提供了机制解释。
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2. **文献名称**：*SIRT4 Has Tumor-Suppressive Activity and Regulates the Cellular Metabolic Response to DNA Damage by Inhibiting Mitochondrial Glutamine Metabolism*
**作者**：Ho, L., Titus, A.S., Banerjee, K.K., et al.
**摘要**：本研究利用重组SIRT4蛋白，证明其通过抑制谷氨酰胺代谢和降低细胞抗氧化能力，抑制肺癌细胞增殖。同时发现SIRT4在DNA损伤后通过调控线粒体代谢途径发挥肿瘤抑制作用。
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3. **文献名称**：*SIRT4 Represses Peroxisome Proliferator-Activated Receptor Alpha Activity to Suppress Hepatic Fatty Acid Oxidation*
**作者**：Laurent, G., de Boer, V.C.J., Finley, L.W.S., et al.
**摘要**：通过重组SIRT4蛋白的功能分析，研究发现SIRT4通过去乙酰化修饰抑制PPARα转录活性，从而下调肝脏脂肪酸氧化相关基因表达，揭示了其在脂代谢平衡中的新机制。
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**备注**：上述文献均为SIRT4功能研究的经典工作，涉及代谢调控、肿瘤抑制及酶活性机制。如需具体发表年份或期刊，可进一步通过作者和标题在PubMed/Google Scholar检索全文。
SIRT4. a member of the sirtuin family of NAD+-dependent deacylases, is a mitochondrial enzyme implicated in metabolic regulation and aging. Initially identified for its role in modulating insulin secretion in pancreatic β-cells, SIRT4 has since been recognized as a multifaceted regulator of mitochondrial metabolism. Unlike other sirtuins (e.g., SIRT1 or SIRT3) that primarily exhibit deacetylase activity, SIRT4 predominantly functions as an ADP-ribosyltransferase and lipoamidase, targeting key enzymes in metabolic pathways such as glutamate dehydrogenase (GDH), pyruvate dehydrogenase complex (PDH), and fatty acid oxidation enzymes. These post-translational modifications enable SIRT4 to suppress catabolic processes under nutrient-rich conditions, thereby fine-tuning energy homeostasis and cellular stress responses.
Recombinant SIRT4 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional SIRT4 for *in vitro* studies. This protein typically retains enzymatic activity and structural integrity, allowing researchers to investigate its substrate specificity, kinetic properties, and interactions with metabolic partners. The development of recombinant SIRT4 has been pivotal in elucidating its role in diseases, including cancer, where it acts as a tumor suppressor by repressing glutamine metabolism, and metabolic disorders like diabetes, where its dysregulation correlates with mitochondrial dysfunction.
Current research leverages recombinant SIRT4 to screen small-molecule modulators for therapeutic applications, aiming to exploit its enzymatic activity to correct metabolic imbalances. Its emerging association with longevity pathways and age-related diseases further underscores its biomedical relevance. However, challenges persist in fully characterizing its diverse enzymatic activities and tissue-specific roles, necessitating advanced recombinant protein tools to bridge mechanistic gaps. Overall, SIRT4 recombinant protein serves as a critical resource for decoding mitochondrial signaling networks and developing targeted metabolic therapies.
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