Recombinant Human FOXA1 protein

中文名： 叉头框蛋白A1(FOXA1)重组蛋白
别名： FOXA1；HNF3A；TCF3A；Hepatocyte nuclear factor 3-alpha

货号: PA1000-8078

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	FOXA1
Uniprot No	P55317
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-472aa
氨基酸序列	MLGTVKMEGHETSDWNSYYADTQEAYSSVPVSNMNSGLGSMNSMNTYMTMNTMTTSGNMTPASFNMSYANPGLGAGLSPGAVAGMPGGSAGAMNSMTAAGVTAMGTALSPSGMGAMGAQQAASMNGLGPYAAAMNPCMSPMAYAPSNLGRSRAGGGGDAKTFKRSYPHAKPPYSYISLITMAIQQAPSKMLTLSEIYQWIMDLFPYYRQNQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWTLHPDSGNMFENGCYLRRQKRFKCEKQPGAGGGGGSGSGGSGAKGGPESRKDPSGASNPSADSPLHRGVHGKTGQLEGAPAPGPAASPQTLDHSGATATGGASELKTPASSTAPPISSGPGALASVPASHPAHGLAPHESQLHLKGDPHYSFNHPFSINNLMSSSEQQHKLDFKAYEQALQYSPYGSTLPASLPLGSASVTTRSPIEPSALEPAYYQGVYSRPVLNTS
预测分子量	56.6 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于FOXA1重组蛋白的3篇参考文献的简要概括(文献标题及核心内容为虚构示例，供参考)：

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1. **标题**: *"Recombinant FOXA1 purification and its role in chromatin remodeling"*

**作者**: Smith J, et al.

**摘要**: 研究报道了利用大肠杆菌系统高效表达并纯化重组FOXA1蛋白的方法，证实其能够特异性结合靶基因启动子区域的保守序列，并在体外实验中促进染色质开放性，为研究FOXA1在基因调控中的作用提供工具。

2. **标题**: *"FOXA1 recombinant protein facilitates cellular reprogramming in breast cancer models"*

**作者**: Lee H, et al.

**摘要**: 通过哺乳动物细胞表达系统获得功能性FOXA1重组蛋白，证明其在乳腺癌细胞中可重塑雌激素受体(ER)的转录活性，揭示了FOXA1通过染色质先驱因子功能驱动癌症表型的关键机制。

3. **标题**: *"Structural analysis of FOXA1-DNA interactions using recombinant protein"*

**作者**: Zhang R, et al.

**摘要**: 利用重组FOXA1蛋白进行X射线晶体学分析，解析了其DNA结合结构域与靶基因结合的分子细节，揭示了特定氨基酸残基对结合亲和力的调控，为靶向FOXA1的药物设计奠定基础。

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(注：以上为模拟内容，实际文献需通过PubMed/Google Scholar等平台以"FOXA1 recombinant protein"等关键词检索。)

背景信息

FOXA1 (Forkhead Box A1) is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved "winged helix" DNA-binding domain. It plays a pivotal role in organ development and differentiation, particularly in tissues such as the prostate, breast, liver, and lungs. FOXA1 acts as a pioneer factor, enabling chromatin remodeling and facilitating the binding of other transcription factors (e.g., estrogen receptor, androgen receptor) to DNA. This function links it to hormone-responsive gene regulation, making it critical in both normal physiology and diseases like cancer.

Recombinant FOXA1 protein is engineered through molecular cloning, typically expressed in *E. coli*, mammalian, or insect cell systems to ensure proper post-translational modifications. The purified protein retains DNA-binding activity and structural integrity, allowing researchers to study its interactions with target genes, co-regulatory proteins, or signaling pathways *in vitro*. Its applications span cancer research (e.g., investigating FOXA1 mutations in hormone-driven tumors), stem cell differentiation studies, and mechanistic analyses of gene regulation.

FOXA1 dysregulation is implicated in multiple cancers, including prostate and breast malignancies, where it may drive oncogenic transcription programs. Recombinant FOXA1 is thus vital for drug discovery, biomarker studies, and functional genomics. Its production under standardized conditions ensures batch-to-batch consistency, enabling reproducible research into its role as a therapeutic target or diagnostic tool.