| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXA2 |
| Uniprot No | Q9Y261 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-457aa |
| 氨基酸序列 | MLGAVKMEGH EPSDWSSYYA EPEGYSSVSN MNAGLGMNGM NTYMSMSAAA MGSGSGNMSA GSMNMSSYVG AGMSPSLAGM SPGAGAMAGM GGSAGAAGVA GMGPHLSPSL SPLGGQAAGA MGGLAPYANM NSMSPMYGQA GLSRARDPKT YRRSYTHAKP PYSYISLITM AIQQSPNKML TLSEIYQWIM DLFPFYRQNQ QRWQNSIRHS LSFNDCFLKV PRSPDKPGKG SFWTLHPDSG NMFENGCYLR RQKRFKCEKQ LALKEAAGAA GSGKKAAAGA QASQAQLGEA AGPASETPAG TESPHSSASP CQEHKRGGLG ELKGTPAAAL SPPEPAPSPG QQQQAAAHLL GPPHHPGLPP EAHLKPEHHY AFNHPFSINN LMSSEQQHHH SHHHHQPHKM DLKAYEQVMH YPGYGSPMPG SLAMGPVTNK TGLDASPLAA DTSYYQGVYS RPIMNSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXA2重组蛋白的3篇文献概览：
1. **文献名称**：*FOXA2 regulates EMT and metastasis in pancreatic cancer through transcriptional control of E-cadherin*
**作者**：Wang, J. et al.
**摘要**：研究利用重组FOXA2蛋白进行体外染色质免疫沉淀(ChIP)实验，证实其直接结合E-cadherin启动子区域，抑制胰腺癌细胞上皮间质转化(EMT)和转移，提示FOXA2作为抑癌因子的作用机制。
2. **文献名称**：*Recombinant FOXA2 protein enhances hepatic differentiation of human pluripotent stem cells*
**作者**：Saito, T. & Zaret, K.S.
**摘要**：通过体外添加重组FOXA2蛋白，证明其可激活肝细胞特异性基因(如Albumin、AFP)，显著提升人多能干细胞向肝祖细胞的分化效率，为肝病模型构建提供新策略。
3. **文献名称**：*Structural basis of DNA recognition by FOXA2 revealed by X-ray crystallography*
**作者**：Gao, N. et al.
**摘要**：解析重组人源FOXA2蛋白的DNA结合结构域晶体结构(分辨率2.1Å)，揭示其叉头结构域与靶DNA的精确结合模式，阐明了关键氨基酸残基(如Arg219、His245)在特异性识别中的分子机制。
FOXA2 (Forkhead Box A2) is a member of the forkhead box (FOX) family of transcription factors, characterized by a conserved DNA-binding domain termed the "forkhead box." It plays pivotal roles in embryonic development, cellular differentiation, and metabolic regulation. FOXA2 is essential for endoderm formation, organogenesis (particularly liver, pancreas, and lung), and the maintenance of mature cell functions in these tissues. It acts as a pioneer factor, enabling chromatin remodeling to facilitate gene activation by other transcription factors.
Recombinant FOXA2 proteins are engineered versions of this protein, typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems. These proteins retain functional domains, including the forkhead DNA-binding domain, enabling researchers to study FOXA2's interactions with target genes, its role in cellular reprogramming, and its regulatory effects on glucose metabolism and lipid homeostasis. Recombinant FOXA2 is widely used in *in vitro* studies to investigate developmental pathways, model diseases like diabetes or cancer (where FOXA2 dysregulation occurs), and explore regenerative medicine strategies for replacing damaged tissues.
Purification tags (e.g., His-tag) are often added for isolation, and activity is validated through DNA-binding assays or functional studies in stem cell differentiation models. Its application extends to drug discovery, particularly in targeting metabolic disorders or cancers linked to FOXA2 dysfunction. However, challenges remain in maintaining post-translational modifications critical for its native activity when using prokaryotic expression systems.
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