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Recombinant Human ICK protein

  • 中文名: 肠细胞激酶(ICK)重组蛋白
  • 别    名: ICK;ICK;KIAA0936;Serine/threonine-protein kinase ICK
货号: PA1000-8071
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ICK
Uniprot No Q9UPZ9
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-632aa
氨基酸序列MNRYTTIRQL GDGTYGSVLL GRSIESGELI AIKKMKRKFY SWEECMNLRE VKSLKKLNHA NVVKLKEVIR ENDHLYFIFE YMKENLYQLI KERNKLFPES AIRNIMYQIL QGLAFIHKHG FFHRDLKPEN LLCMGPELVK IADFGLAREI RSKPPYTDYV STRWYRAPEV LLRSTNYSSP IDVWAVGCIM AEVYTLRPLF PGASEIDTIF KICQVLGTPK KTDWPEGYQL SSAMNFRWPQ CVPNNLKTLI PNASSEAVQL LRDMLQWDPK KRPTASQALR YPYFQVGHPL GSTTQNLQDS EKPQKGILEK AGPPPYIKPV PPAQPPAKPH TRISSRQHQA SQPPLHLTYP YKAEVSRTDH PSHLQEDKPS PLLFPSLHNK HPQSKITAGL EHKNGEIKPK SRRRWGLISR STKDSDDWAD LDDLDFSPSL SRIDLKNKKR QSDDTLCRFE SVLDLKPSEP VGTGNSAPTQ TSYQRRDTPT LRSAAKQHYL KHSRYLPGIS IRNGILSNPG KEFIPPNPWS SSGLSGKSSG TMSVISKVNS VGSSSTSSSG LTGNYVPSFL KKEIGSAMQR VHLAPIPDPS PGYSSLKAMR PHPGRPFFHT QPRSTPGLIP RPPAAQPVHG RTDWASKYAS RR
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ICK重组蛋白的参考文献示例(注:以下为模拟示例,实际文献可能需要通过学术数据库查询):

1. **标题**: "Expression and Functional Characterization of Recombinant Human Intestinal Cell Kinase (ICK) in Escherichia coli"

**作者**: Smith J, Doe R, Lee M

**摘要**: 本研究成功在大肠杆菌中表达并纯化重组人ICK蛋白,证实其具有激酶活性,并验证了其在细胞周期调控及纤毛形成中的功能。

2. **标题**: "Crystal Structure of Recombinant ICK Reveals Insights into Its Role in Ciliopathy Disorders"

**作者**: Chen X, Wang L, Tanaka T

**摘要**: 通过解析重组ICK蛋白的晶体结构,揭示了其与纤毛病相关突变的作用机制,为疾病治疗提供了结构生物学基础。

3. **标题**: "High-Yield Production of ICK Recombinant Protein Using Baculovirus-Insect Cell System"

**作者**: Gupta S, Zhang Y, Kumar P

**摘要**: 开发了一种基于杆状病毒-昆虫细胞系统的高效ICK重组蛋白表达方法,适用于大规模功能研究与药物开发。

4. **标题**: "ICK Recombinant Protein Modulates Wnt/β-catenin Signaling in Developmental Models"

**作者**: Brown K, Johnson A, Garcia E

**摘要**: 研究发现重组ICK蛋白通过调控Wnt/β-catenin信号通路影响胚胎发育,为发育生物学提供了新的分子机制解释。

建议通过PubMed或Google Scholar以“recombinant ICK protein”、“ICK kinase expression”等关键词检索获取真实文献。

背景信息

ICK (Intestinal Cell Kinase), also known as MAK-like protein kinase, is a serine/threonine protein kinase belonging to the cyclin-dependent kinase (CDK)-related kinase family. Initially identified for its role in intestinal cell differentiation, ICK has gained attention for its critical involvement in ciliogenesis, cell cycle regulation, and embryonic development. It shares structural homology with CDKs but exhibits distinct functional characteristics, particularly in phosphorylating substrates associated with primary cilia formation and maintenance.

The recombinant ICK protein is engineered using genetic cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper post-translational modifications. This recombinant form retains the kinase's enzymatic activity, enabling researchers to study its molecular mechanisms in vitro. ICK interacts with key ciliary proteins like KIF3A and regulates the Hedgehog signaling pathway, making it essential for understanding cilia-related disorders (ciliopathies) such as polycystic kidney disease, retinal degeneration, and skeletal abnormalities.

Recent studies highlight ICK's dual role in promoting cilia growth while suppressing cell cycle progression, a balance crucial for tissue homeostasis. Dysregulation of ICK has been linked to cancer proliferation, neurodevelopmental defects, and metabolic disorders. Recombinant ICK serves as a vital tool for drug discovery, particularly in screening kinase inhibitors or modulators for therapeutic interventions. Its structural analysis (e.g., ATP-binding pocket mapping) further aids in designing targeted therapies. Ongoing research aims to clarify ICK's tissue-specific functions and its potential as a biomarker or therapeutic target in human diseases.

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