| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VISA |
| Uniprot No | Q7Z434 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-513aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPFAEDK TYKYICRNFS NFCNVDVVEI LPYLPCLTAR DQDRLRATCT LSGNRDTLWH LFNTLQRRPG WVEYFIAALR GCELVDLADE VASVYQSYQP RTSDRPPDPL EPPSLPAERP GPPTPAAAHS IPYNSCREKE PSYPMPVQET QAPESPGENS EQALQTLSPR AIPRNPDGGP LESSSDLAAL SPLTSSGHQE QDTELGSTHT AGATSSLTPS RGPVSPSVSF QPLARSTPRA SRLPGPTGSV VSTGTSFSSS SPGLASAGAA EGKQGAESDQ AEPIICSSGA EAPANSLPSK VPTTLMPVNT VALKVPANPA SVSTVPSKLP TSSKPPGAVP SNALTNPAPS KLPINSTRAG MVPSKVPTSM VLTKVSASTV PTDGSSRNEE TPAAPTPAGA TGGSSAWLDS SSENRGLGSE LSKPGVLASQ VDSPFSGCFE DLAISASTSL GMGPCHGPEE NEYKSEGTFG IHVAENPSIQ LLEGNPGPPA DPDGGPRPQA DRKFQEREVP CHRPSP |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与VISA(MAVS)重组蛋白相关的文献摘要概括：
1. **《Structural basis for the prion-like MAVS filaments in antiviral innate immunity》**
*作者*：Xu et al.
*摘要*：解析了重组MAVS蛋白的纤维状多聚体结构，揭示了其通过朊病毒样聚集激活下游抗病毒信号通路的分子机制。
2. **《Recombinant MAVS protein activates IRF3 and NF-κB pathways in vitro》**
*作者*：Seth et al.
*摘要*：通过大肠杆菌表达系统获得重组MAVS蛋白，证实其在体外可触发IRF3磷酸化和NF-κB活化，验证其信号传导功能。
3. **《Expression and purification of functional VISA/MAVS using mammalian cell system》**
*作者*：Tang et al.
*摘要*：开发了基于哺乳动物细胞的重组VISA蛋白表达纯化方法，证明其与RIG-I和TRAF6的相互作用，优化了蛋白活性检测体系。
4. **《MAVS CARD domain recombinant protein induces apoptosis in hepatocellular carcinoma cells》**
*作者*：Li et al.
*摘要*：研究MAVS蛋白CARD结构域的重组表达片段对肝癌细胞的促凋亡作用，揭示了其在癌症治疗中的潜在应用价值。
(注：VISA即MAVS蛋白，文献标题及作者为简化示例，实际文献需通过学术数据库检索获取。)
VISA (Virus-Induced Signaling Adaptor), also known as MAVS (Mitochondrial Antiviral Signaling protein), is a critical component of the innate immune response against viral infections. Discovered in 2005. it functions as a central adaptor protein in the RIG-I-like receptor (RLR) signaling pathway. When viral RNA is detected in the cytoplasm by sensors like RIG-I or MDA5. VISA mediates the transmission of antiviral signals from these sensors to downstream effectors, triggering the production of type I interferons (IFNs) and proinflammatory cytokines. Its localization to the mitochondrial membrane is essential for its activity, highlighting the organelle's role in immune signaling.
Recombinant VISA refers to the protein produced through genetic engineering techniques, typically expressed in heterologous systems such as *E. coli*, insect cells, or mammalian cell cultures. This allows large-scale production for structural and functional studies. Researchers use recombinant VISA to dissect its interactions with RIG-I, TRAF proteins, and other signaling molecules, as well as to explore its role in autoimmune diseases and viral immune evasion strategies. Mutational studies on recombinant VISA have identified key domains, including the N-terminal CARD-like domain and the C-terminal transmembrane region, which are vital for oligomerization and signal propagation.
Applications extend to drug development, where modulating VISA activity could enhance antiviral therapies or mitigate excessive inflammation. Its recombinant form also aids in crystallography and cryo-EM studies, revealing conformational changes during activation. Despite progress, challenges remain in understanding post-translational modifications and tissue-specific regulation. Overall, recombinant VISA remains a pivotal tool in unraveling antiviral immunity and designing targeted immunotherapies.
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