| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFP3 |
| Uniprot No | Q96NJ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-502 aa |
| 活性数据 | MGTENKEVIPKEEISEESEPHGSLLEKFPKVVYQGHEFGAGCEEDMLEGHSRESMEEVIEQMSPQERDFPSGLMIFKKSPSSEKDRENNESERGCSPSPNLVTHQGDTTEGVSAFATSGQNFLEILESNKTQRSSVGEKPHTCKECGKAFNQNSHLIQHMRVHSGEKPFECKECGKTFGTNSSLRRHLRIHAGEKPFACNECGKAFIQSSHLIHHHRIHTGERPYKCEECGKAFSQNSALILHQRIHTGEKPYECNECGKTFRVSSQLIQHQRIHTEERYHECNECGKAFKHSSGLIRHQKIHTGEKPYLCNECGKGFGQSSELIRHQRIHTGDKPYECNECGKTFGQNSEIIRHIRIHTGEKPYVCKECGKAFRGNSELLRHERIHTGEKPYECFECGKAFRRTSHLIVHQRIHTGEKPHQCNECARTFWDNSELLLHQKIHIGEKPYECSECEKTFSQHSQLIIHQRIHTGEKPYECQECQKTFSRSSHLLRHQSVHCME |
| 分子量 | 84.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人ZFP3蛋白的虚构参考文献示例(基于领域知识推测，实际文献需通过数据库验证)：
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1. **文献名称**：Structural and Functional Analysis of Recombinant Human ZFP3 Protein
**作者**：Zhang Y. et al. (2021)
**摘要**：报道了重组人ZFP3蛋白的晶体结构，揭示其锌指结构域与靶DNA序列的特异性结合模式，为基因编辑工具设计提供基础。
2. **文献名称**：ZFP3-Mediated Transcriptional Repression in Cancer Cell Proliferation
**作者**：Li H. et al. (2019)
**摘要**：通过体外表达重组人ZFP3蛋白，证明其通过抑制致癌基因转录减缓肿瘤生长，提示其作为潜在治疗靶点。
3. **文献名称**：High-Yield Production of Recombinant ZFP3 in E. coli and Its Application in Gene Regulation
**作者**：Wang J. et al. (2018)
**摘要**：开发了重组ZFP3蛋白在大肠杆菌中的高效表达及纯化方法，验证其在哺乳动物细胞中调控报告基因表达的功能。
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**说明**：以上内容为模拟示例，实际文献需查阅PubMed、Google Scholar等数据库，使用关键词"recombinant ZFP3"或"Zinc Finger Protein 3"进行检索。如研究较少，可扩展至锌指蛋白家族(如ZFP14、ZNFs)或相关功能研究。
ZFP3. a member of the zinc finger protein (ZFP) family, is a transcription factor characterized by its conserved Cys2/His2-type zinc finger domains, which enable sequence-specific DNA binding. These structural motifs facilitate interactions with target gene promoters or regulatory elements, playing roles in transcriptional regulation, chromatin remodeling, and cellular processes such as differentiation, proliferation, and apoptosis. Human ZFP3 is implicated in early embryonic development and stem cell maintenance, with studies suggesting its involvement in epigenetic modifications and cell fate determination.
Recombinant human ZFP3 protein is typically engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. It serves as a vital tool for elucidating ZFP3's molecular mechanisms, including its DNA-binding specificity, interactions with co-regulators, and influence on gene networks. Researchers utilize recombinant ZFP3 in *in vitro* assays (e.g., EMSA, ChIP) and cell-based studies to explore its role in diseases like cancer, where dysregulation of zinc finger proteins may contribute to oncogenesis or tumor suppression. Additionally, its potential application in synthetic biology, such as designing custom transcription factors for gene therapy, highlights its therapeutic relevance. Despite progress, functional redundancies within the ZFP family and context-dependent activity necessitate further investigation to fully harness its biomedical potential.
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