| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF12 |
| Uniprot No | P17014 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697 aa |
| 活性数据 | MNKSLGPVSF KDVAVDFTQE EWQQLDPEQK ITYRDVMLEN YSNLVSVGYH IIKPDVISKL EQGEEPWIVE GEFLLQSYPD EVWQTDDLIE RIQEEENKPS RQTVFIETLI EERGNVPGKT FDVETNPVPS RKIAYKNSLC DSCEKCLTSV SEYISSDGSY ARMKADECSG CGKSLLHIKL EKTHPGDQAY EFNQNGEPYT LNEESLYQKI RILEKPFEYI ECQKAFQKDT VFVNHMEEKP YKWNGSEIAF LQMSDLTVHQ TSHMEMKPYE CSECGKSFCK KSKFIIHQRT HTGEKPYECN QCGKSFCQKG TLTVHQRTHT GEKPYECNEC GKNFYQKLHL IQHQRTHSGE KPYECSYCGK SFCQKTHLTQ HQRTHSGERP YVCHDCGKTF SQKSALNDHQ KIHTGVKLYK CSECGKCFCR KSTLTTHLRT HTGEKPYECN ECGKFFSRLS YLTVHYRTHS GEKPYECNEC GKTFYLNSAL MRHQRVHTGE KPYECNECGK LFSQLSYLTI HHRTHSGVKP YECSECGKTF YQNSALCRHR RIHKGEKPYE CYICGKFFSQ MSYLTIHHRI HSGEKPYECS ECGKTFCQNS ALNRHQRTHT GEKAYECYEC GKCFSQMSYL TIHHRIHSGE KPFECNECGK AFSRMSYLTV HYRTHSGEKP YECTECGKKF YHKSAFNSHQ RIHRRGNMNV IDVGRLL |
| 分子量 | 81.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF12蛋白的示例参考文献(注：由于文献信息可能涉及版权问题，以下内容为模拟文献示例)：
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1. **文献名称**：*Structural characterization of recombinant human ZNF12 and its DNA-binding properties*
**作者**：Smith A, et al. (2015)
**摘要**：本研究通过大肠杆菌表达系统纯化重组人ZNF12蛋白，结合X射线晶体学解析其三维结构，发现其锌指结构域对特定DNA序列的亲和性，揭示了其在基因调控中的潜在作用。
2. **文献名称**：*ZNF12 suppresses tumor metastasis via transcriptional regulation of EMT markers*
**作者**：Zhang L, et al. (2018)
**摘要**：通过重组ZNF12蛋白的体外功能实验，发现其可抑制癌症细胞的侵袭和转移。机制研究表明，ZNF12通过结合上皮间质转化(EMT)相关基因启动子区域抑制其表达。
3. **文献名称**：*Optimization of recombinant human ZNF12 expression in mammalian cells for functional studies*
**作者**：Lee J, et al. (2020)
**摘要**：探讨了哺乳动物细胞表达系统中重组ZNF12蛋白的优化策略，包括密码子优化和标签设计，提高了蛋白可溶性与稳定性，为后续生化分析奠定基础。
4. **文献名称**：*Functional analysis of ZNF12 in neuronal differentiation using recombinant protein delivery*
**作者**：Chen X, et al. (2017)
**摘要**：将重组ZNF12蛋白导入神经前体细胞，发现其通过调控靶基因(如Sox2)的表达促进神经元分化，提示其在神经发育中的潜在功能。
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**注意**：以上文献为示例性质，实际研究中建议通过PubMed、Web of Science等数据库搜索关键词“ZNF12”或“recombinant ZNF12 protein”获取真实文献。若需具体文献支持，可提供更详细的研究方向(如结构、功能、疾病关联等)。
Zinc finger protein 12 (ZNF12), also known as Krox-20 or EGR2. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which plays pivotal roles in transcriptional regulation and chromatin remodeling. Structurally, ZNF12 contains multiple C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, along with a KRAB domain at the N-terminus known to recruit corepressor complexes, thereby suppressing gene expression. It is ubiquitously expressed in human tissues and implicated in critical biological processes, including cell differentiation, proliferation, and apoptosis.
Recombinant human ZNF12 protein is typically produced using bacterial or mammalian expression systems, enabling studies of its molecular functions and interactions. Research highlights its involvement in embryonic development, neural differentiation, and immune regulation. Dysregulation of ZNF12 has been linked to cancers, neurodegenerative diseases, and autoimmune disorders, making it a potential therapeutic target. Recombinant ZNF12 serves as a tool to investigate DNA-protein interactions, screen for small-molecule inhibitors, and explore epigenetic modulation mechanisms. Recent studies also suggest its role in maintaining genomic stability and mediating stress responses, expanding its relevance in both basic and translational research.
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