| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF232 |
| Uniprot No | Q9UNY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-417 aa |
| 活性数据 | MAVSLTAAET LALQGTQGQE KMMMMGPKEE EQSCEYETRL PGNHSTSQEI FRQRFRHLRY QETPGPREAL SQLRVLCCEW LRPEKHTKEQ ILEFLVLEQF LTILPEELQS WVRGHHPKSG EEAVTVLEDL EKGLEPEPQV PGPAHGPAQE EPWEKKESLG AAQEALSIQL QPKETQPFPK SEQVYLHFLS VVTEDGPEPK DKGSLPQPPI TEVESQVFSE KLATDTSTFE ATSEGTLELQ QRNPKAERLR WSPAQEESFR QMVVIHKEIP TGKKDHECSE CGKTFIYNSH LVVHQRVHSG EKPYKCSDCG KTFKQSSNLG QHQRIHTGEK PFECNECGKA FRWGAHLVQH QRIHSGEKPY ECNECGKAFS QSSYLSQHRR IHSGEKPFIC KECGKAYGWC SELIRHRRVH ARKEPSH |
| 分子量 | 50.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性示例(真实文献需通过学术数据库查询)：
1. **文献名称**："Cloning and Prokaryotic Expression of Human ZNF232 Protein"
**作者**：Wang et al.
**摘要**：成功构建了人ZNF232的原核表达载体，优化后获得可溶性重组蛋白，并通过Western blot验证其表达，为功能研究提供基础。
2. **文献名称**："ZNF232 Represses AP-1 Activity via Direct DNA Binding"
**作者**：Liu et al.
**摘要**：在HEK293细胞中表达重组ZNF232.发现其通过结合AP-1启动子区抑制转录活性，暗示其在肿瘤抑制中的潜在作用。
3. **文献名称**："Structural Insights into ZNF232-DNA Interaction by Recombinant Protein Crystallography"
**作者**：Johnson et al.
**摘要**：利用昆虫细胞系统表达并结晶重组ZNF232锌指结构域，揭示其与靶DNA序列的精确结合模式。
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**提示**：实际文献建议通过PubMed/Google Scholar搜索关键词：
`"recombinant ZNF232"`、`"ZNF232 protein expression"`、`"ZNF232 function"`。部分研究可能涵盖基因克隆、转录调控或疾病关联方向。
Zinc finger protein 232 (ZNF232), also known as recombinant human ZNF232 when produced via biotechnological methods, is a member of the zinc finger protein family characterized by conserved Cys₂His₂ (C2H2) zinc finger domains. These structural motifs enable sequence-specific DNA binding, positioning ZNF232 as a transcription regulator involved in gene expression modulation. The protein is localized primarily in the nucleus and has been implicated in cellular processes including proliferation, differentiation, and apoptosis. Its genomic structure comprises multiple exons encoding tandem zinc finger repeats, reflecting its evolutionary conservation across species.
Research suggests ZNF232 interacts with specific promoter regions to either activate or repress target genes. Notably, it has been associated with stress response pathways, particularly oxidative stress, potentially through regulating antioxidant enzyme expression. Aberrant ZNF232 expression has been observed in pathologies such as cancers, neurodegenerative disorders, and metabolic syndromes. In some cancers, ZNF232 may act as a tumor suppressor by inhibiting cell cycle progression or promoting apoptosis, though its context-dependent roles require further validation.
Recombinant ZNF232 is typically expressed in bacterial or mammalian systems for functional studies. Current research focuses on elucidating its molecular mechanisms, physiological partners, and therapeutic potential. Advances in CRISPR/Cas9 editing and omics technologies are accelerating its exploration as a diagnostic marker or drug target. Despite progress, comprehensive understanding of its tissue-specific functions and post-translational modifications remains limited, warranting deeper investigation.
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