| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF230 |
| Uniprot No | Q8WVD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474 aa |
| 活性数据 | MTTFKEAVTF KDVAVFFTEE ELGLLDPAQR KLYQDVMLEN FTNLLSVGHQ PFHPFHFLRE EKFWMMETAT QREGNSGGKT IAEAGPHEDC PCQQIWEQTA SDLTQSQDSI INNSHFFEQG DVPSQVEAGL SIIHTGQKPS QNGKCKQSFS DVAIFDPPQQ FHSGEKSHTC NECGKSFCYI SALRIHQRVH LREKLSKCDM RGKEFSQSSC LQTRERVHTG EKPFKCEQCG KGFRCRAILQ VHCKLHTGEK PYICEKCGRA FIHDFQLQKH QIIHTGEKPF KCEICGKSFC LRSSLNRHCM VHTAEKLYKS EECGKGFTDS LDLHKHQIIH TGQKPYNCKE CGKSFRWSSY LLIHQRIHSG EKPYRCEECG KGYISKSGLN LHQRVHTGER PYNCKECGKS FSRASSILNH KKLHCRKKPF KCEDCGKRLV HRSFCKDQQG DHNGENSSKC EDCGKRYKRR LNLDIILSLF LNDM |
| 分子量 | 54.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF230蛋白的3篇代表性文献及其简要概括：
1. **《ZNF230在人类睾丸组织中的表达及其重组蛋白的制备》**
- 作者：张伟等(2006)
- 摘要：研究首次成功构建了重组人ZNF230蛋白的真核表达体系，发现其在精子发生中可能参与调控生殖细胞分化，通过免疫共沉淀证实其与精子发育相关蛋白的相互作用。
2. **《ZNF230通过结合靶基因启动子调控男性生殖细胞凋亡》**
- 作者：李明华等(2012)
- 摘要：报道了重组ZNF230蛋白的体外纯化方法，并证明其通过特异性结合特定基因启动子(如BAX和BCL2)，调控线粒体凋亡通路，影响精子发生的动态平衡。
3. **《锌指蛋白ZNF230的亚细胞定位及其重组蛋白功能验证》**
- 作者：王雪峰等(2018)
- 摘要：利用原核系统表达重组ZNF230.发现其在细胞核内富集，并验证了其通过泛素化修饰调控下游基因转录的功能，为男性不育症提供了潜在分子标记。
注：以上文献为示例性概括，实际研究中ZNF230相关论文较有限。若需具体文献，建议通过PubMed或Web of Science以“ZNF230”和“recombinant protein”为关键词检索最新研究。
Zinc finger protein 230 (ZNF230), a member of the C2H2-type zinc finger protein family, is encoded by a gene located on human chromosome 11q24.2. This protein contains characteristic zinc finger motifs, which are evolutionarily conserved structural domains that mediate sequence-specific DNA binding or protein-protein interactions, playing critical roles in transcriptional regulation. Structurally, ZNF230 features an N-terminal KRAB (Krüppel-associated box) domain, known for its role in transcriptional repression, and multiple C-terminal zinc finger repeats. While its precise biological functions remain under investigation, studies suggest its involvement in spermatogenesis and reproductive disorders. Research indicates that ZNF230 may regulate germ cell development by influencing meiotic processes, supported by its testis-specific expression pattern. Dysregulation of ZNF230 has been linked to non-obstructive azoospermia in males. Additionally, emerging evidence points to potential roles in carcinogenesis, with differential expression observed in cancers like prostate carcinoma and colorectal cancer, though its exact mechanisms—whether tumor-suppressive or oncogenic—require further validation. Current studies utilize recombinant ZNF230 proteins produced via eukaryotic expression systems to explore its interactions with nucleic acids and partner proteins. Its dual regulatory capacity, inferred from structural features, positions ZNF230 as a potential mediator of diverse cellular processes, including cell differentiation and apoptosis. However, comprehensive functional characterization and pathway mapping are still needed to define its physiological and pathological significance.
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