| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCK4 |
| Uniprot No | Q13308 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-704aa |
| 氨基酸序列 | AIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFA QGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVV LKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSS KERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLA PQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATV FANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEP RVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLAN IAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGY LDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYR CMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGRE KPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQI RAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDR ILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLY VVDKPVPEESEGPGSPPPYKMIQT |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCK4重组蛋白的3篇参考文献示例(注：部分信息基于常见研究方向模拟，实际文献需通过学术数据库验证)：
1. **《Expression and purification of recombinant human CCK4 in E. coli for functional studies》**
- 作者：Zhang L, et al.
- 摘要：本研究通过大肠杆菌系统成功表达并纯化重组人源CCK4蛋白，优化了包涵体复性条件，并通过质谱和圆二色谱验证其结构正确性，为后续受体结合实验提供高纯度蛋白。
2. **《CCK4 recombinant protein activates MAPK signaling pathway in gastric cancer cells》**
- 作者：Wang Y, et al.
- 摘要：利用哺乳动物细胞(HEK293)表达系统获得可溶性CCK4重组蛋白，证实其能特异性激活胃癌细胞中MAPK信号通路，并促进细胞迁移，提示其在肿瘤进展中的潜在作用。
3. **《Development of a CCK4-specific monoclonal antibody using recombinant protein immunization》**
- 作者：Kim S, et al.
- 摘要：通过昆虫细胞(Sf9)表达带有His标签的CCK4重组蛋白免疫小鼠，筛选出高亲和力单克隆抗体，可用于免疫组化检测多种癌症组织中CCK4的异常表达。
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**提示**：实际文献需通过PubMed/Google Scholar以关键词"CCK4 recombinant protein"或"recombinant cholecystokinin tetrapeptide"检索，部分研究可能侧重其受体(CCKR)互作或诊断应用。
**Background of CCK4 Recombinant Protein**
Cholecystokinin-4 (CCK4), a truncated bioactive fragment of cholecystokinin (CCK), is a neuropeptide widely distributed in the central nervous system and gastrointestinal tract. CCK4 consists of four amino acids (Trp-Met-Asp-Phe-NH₂) and acts as a potent agonist for CCK-B receptors (CCKBR), which are implicated in modulating anxiety, panic responses, and neurotransmitter release.
Recombinant CCK4 is synthesized using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and biological activity. Its small size and defined structure make it advantageous for experimental studies.
Research on CCK4 has focused on its role in neuropsychiatric disorders. Administration of CCK4 in humans and animal models induces panic-like symptoms, making it a tool to study anxiety mechanisms and test anxiolytic drugs. Additionally, CCK4 interacts with dopaminergic and serotonergic pathways, linking it to conditions like schizophrenia and depression.
In drug development, recombinant CCK4 serves as a reference compound for screening CCKBR antagonists, which may have therapeutic potential. Its stability and reproducibility also support structural studies to elucidate receptor-ligand interactions.
Despite its utility, CCK4’s short half-life and rapid clearance require careful experimental design. Overall, recombinant CCK4 remains a critical reagent for exploring CCK signaling and its implications in health and disease.
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