| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF71 |
| Uniprot No | Q9NQZ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-489 aa |
| 活性数据 | MKELDPKNDISEDKLSVVGEATGGPTRNGARGPGSEGVWEPGSWPERPRGDAGAEWEPLGIPQGNKLLGGSVPACHELKAFANQGCVLVPPRLDDPTEKGACPPVRRGKNFSSTSDLSKPPMPCEEKKTYDCSECGKAFSRSSSLIKHQRIHTGEKPFECDTCGKHFIERSSLTIHQRVHTGEKPYACGDCGKAFSQRMNLTVHQRTHTGEKPYVCDVCGKAFRKTSSLTQHERIHTGEKPYACGDCGKAFSQNMHLIVHQRTHTGEKPYVCPECGRAFSQNMHLTEHQRTHTGEKPYACKECGKAFNKSSSLTLHQRNHTGEKPYVCGECGKAFSQSSYLIQHQRFHIGVKPFECSECGKAFSKNSSLTQHQRIHTGEKPYECYICKKHFTGRSSLIVHQIVHTGEKPYVCGECGKAFSQSAYLIEHQRIHTGEKPYRCGQCGKSFIKNSSLTVHQRIHTGEKPYRCGECGKTFSRNTNLTRHLRIHT |
| 分子量 | 80.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF71蛋白的3篇代表性文献的简要信息(部分为假设性示例供参考)：
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1. **文献名称**：*"ZNF71 regulates tumor progression through epigenetic silencing of tumor suppressor genes in colorectal cancer"*
**作者**：Zhang L, et al. (2020)
**摘要**：本研究揭示了ZNF71通过结合特定基因启动子区域并招募组蛋白甲基化修饰复合物，表观沉默多个抑癌基因(如CDKN1A)，进而促进结直肠癌细胞增殖和转移。实验通过重组人ZNF71蛋白验证其DNA结合活性，并证明其在肿瘤中的高表达与患者不良预后相关。
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2. **文献名称**：*"Structural and functional analysis of the zinc finger domains of human ZNF71 protein"*
**作者**：Kim J, Park SM. (2018)
**摘要**：通过X射线晶体学解析了ZNF71的锌指结构域三维结构，发现其C端锌指模体具有独特的DNA结合特性。利用重组表达的ZNF71蛋白进行凝胶迁移实验(EMSA)，证实其优先识别富含GC碱基对的DNA序列，为解析其靶基因调控机制提供结构基础。
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3. **文献名称**：*"Recombinant ZNF71 protein as a potential diagnostic marker for gastric cancer"*
**作者**：Wang Y, et al. (2022)
**摘要**：本研究开发了基于大肠杆菌表达系统的重组ZNF71蛋白，并验证其在血清中的自身抗体水平可作为胃癌早期诊断标志物。通过ELISA检测发现，胃癌患者血清中抗ZNF71 IgG滴度显著高于健康对照组，提示其临床转化潜力。
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注：以上文献信息结合了真实研究方向和部分虚构内容，如需实际文献建议通过PubMed或Web of Science检索关键词"ZNF71"或"Zinc Finger Protein 71"。
Zinc finger protein 71 (ZNF71), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family, is a transcription factor implicated in gene regulation and chromatin remodeling. Structurally, it features multiple C2H2-type zinc finger motifs, which enable sequence-specific DNA binding, and a KRAB domain at its N-terminus that often mediates interactions with co-repressors to suppress transcription. ZNF71 is thought to participate in developmental processes, cellular differentiation, and the maintenance of genomic stability, though its precise biological roles remain less characterized compared to other KZNF proteins.
Recombinant human ZNF71 protein is engineered for experimental studies, typically produced using expression systems like *E. coli* or mammalian cells, followed by purification to ensure functionality. Researchers utilize this protein to investigate its DNA-binding specificity, regulatory targets, and interactions with epigenetic modifiers (e.g., TRIM28/KAP1). Studies suggest potential links to diseases, including certain cancers, where aberrant ZNF71 expression may influence oncogenic pathways or tumor suppression. Its role in viral defense mechanisms, a hallmark of some KZNFs, is also under exploration.
As a research tool, recombinant ZNF71 aids in elucidating the molecular basis of gene silencing, chromatin dynamics, and disease mechanisms, offering insights for therapeutic targeting. Further work is needed to map its full regulatory network and validate its functional significance in health and disease.
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