| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF709 |
| Uniprot No | Q8N972 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-641 aa |
| 活性数据 | MDSVVFEDVAVNFTQEEWALLGPSQKKLYRDVMQETFVNLASIGENWEEKNIEDHKNQGRKLRSHMVERLCERKEGSQFGETISQTPNPKPNKKTFTRVKPYECSVCGKDYMCHSSLNRHMRSHTEHRSYEYHKYGEKSYECKECGKRFSFRSSFRIHERTHTGEKPYKCKQCGKAFSWPSSFQIHERTHTGEKPYECKECGKAFIYHTTFRGHMRMHTGEKPYKCKECGKTFSHPSSFRNHERTHSGEKPYECKQCGKAFRYYQTFQIHERTHTGEKPYQCKQCGKALSCPTSFRSHERIHTGEKPYKCKKCGKAFSFPSSFRKHERIHTGEKPYDCKECGKAFISLPSYRRHMIMHTGNGPYKCKECGKAFDCPSSFQIHERTHTGEKPYECKQCGKAFSCSSSFRMHERTHTGEKPHECKQCGKAFSCSSSVRIHERTHTGEKPYECKQCGKAFSCSSSFRMHERIHTGEKPYECKQCGKAFSFSSSFRMHERTHTGEKPYECKQCGKAFSCSSSFRMHERTHTGEKPYECKQCGKAFSCSSSIRIHERTHTGEKPYECKQCGKAFSCSSSVRMHERTHTGVKPYECKQCDKAFSCSRSFRIHERTHTGEKPYACQQCGKAFKCSRSFRIHERVHSGE |
| 分子量 | 101.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人ZNF709蛋白的假设参考文献示例(由于真实文献数据有限，以下内容为基于该领域研究模式的合理推测，仅作参考)：
1. **《Functional characterization of recombinant human ZNF709 in transcriptional regulation》**
- **作者**: Chen, L., et al. (2022)
- **摘要**: 研究报道了在大肠杆菌中成功表达并纯化重组人ZNF709蛋白，通过体外DNA结合实验和基因表达分析，发现其通过与特定启动子区域结合抑制肿瘤相关基因(如p53)的转录，提示其在癌症中的潜在调控作用。
2. **《ZNF709 interacts with HDAC complexes to modulate neuronal gene silencing》**
- **作者**: Smith, J.R., et al. (2020)
- **摘要**: 利用重组ZNF709蛋白的体外结合实验和免疫共沉淀技术，证明其与组蛋白去乙酰化酶(HDAC)复合物互作，参与神经元分化过程中的表观遗传调控，可能与阿尔茨海默病相关基因沉默有关。
3. **《Structural analysis of the zinc finger domains in human ZNF709》**
- **作者**: Wang, Y., et al. (2019)
- **摘要**: 通过X射线晶体学解析了重组ZNF709蛋白的锌指结构域三维结构，揭示了其独特的C2H2锌指模体排列方式，为理解其靶向DNA结合的分子机制提供了结构基础。
4. **《Overexpression of recombinant ZNF709 promotes cell proliferation in hepatocellular carcinoma》**
- **作者**: Zhang, H., et al. (2021)
- **摘要**: 在肝癌细胞系中过表达重组ZNF709蛋白，发现其通过激活Wnt/β-catenin信号通路显著促进癌细胞增殖和迁移，提示其作为肝癌治疗潜在靶点的可能性。
---
**注**：以上文献为根据锌指蛋白家族(ZNF)常见研究方向模拟的假设性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
Zinc finger protein 709 (ZNF709) is a member of the zinc finger protein family, which is characterized by conserved C2H2-type zinc finger motifs that facilitate DNA or protein interactions. As a transcription factor, ZNF709 is implicated in transcriptional regulation, chromatin remodeling, and epigenetic modifications. The human ZNF709 gene, located on chromosome 19 (19p13.2), encodes a multi-domain protein containing tandem zinc finger repeats, a KRAB (Krüppel-associated box) domain, and a SCAN domain. The KRAB domain often mediates transcriptional repression by recruiting co-repressor complexes, while the SCAN domain facilitates protein-protein interactions, suggesting ZNF709’s role in gene silencing or regulatory networks.
Recombinant human ZNF709 protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form enables investigations into DNA-binding specificity, interaction partners, and regulatory mechanisms. Studies suggest ZNF709 may influence cell proliferation, differentiation, and stress responses, with dysregulation linked to cancers, neurodevelopmental disorders, and autoimmune diseases. For example, aberrant ZNF709 expression has been observed in certain tumors, potentially affecting oncogenic pathways or genomic stability. Researchers utilize recombinant ZNF709 to dissect its role in disease pathogenesis, screen for therapeutic targets, or engineer gene-editing tools leveraging its DNA-binding properties. Further exploration of ZNF709 could unveil its contributions to cellular homeostasis and disease, offering insights for precision medicine strategies.
×
