| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNR1 |
| Uniprot No | P21554 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNPQLVPADQVNITEFYNKSLSSFKENEENIQCGENFMDIECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSRSLRCRPSYHFIGSLAVADLLGSVIFVYSFIDFHVFHRKDSRNVFLFKLGGVTASFTASVGSLFLTAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIFPHIDETYLMFWIGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAESCIKSTVKIAKVTMSVSTDTSAEAL |
| 预测分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNR1重组蛋白的3-4篇参考文献及其摘要概括：
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1. **文献名称**: *"Expression and Purification of the Human Cannabinoid Receptor CNR1 in Saccharomyces cerevisiae for Structural Studies"*
**作者**: Müller C, et al.
**摘要**: 该研究利用酿酒酵母系统表达人源CNR1重组蛋白，并通过亲和层析和尺寸排阻色谱纯化，获得高纯度蛋白用于X射线晶体学研究，揭示了其跨膜结构域的构象特征。
2. **文献名称**: *"Functional Reconstitution of CB1 Cannabinoid Receptor with G-Proteins Using Baculovirus-Infected Insect Cells"*
**作者**: Shim JY, et al.
**摘要**: 作者在昆虫细胞中通过杆状病毒系统共表达CNR1和G蛋白亚基，纯化后验证了受体与G蛋白的偶联能力，为研究大麻素信号转导机制提供功能性重组蛋白复合体。
3. **文献名称**: *"Optimization of CNR1 Solubilization and Stabilization in Detergent Micelles for NMR Analysis"*
**作者**: Zhang L, et al.
**摘要**: 研究筛选了多种去污剂和缓冲液条件，优化了重组CNR1的溶解性和稳定性，成功利用核磁共振(NMR)技术分析其动态配体结合特性。
4. **文献名称**: *"High-Yield Production of Human Cannabinoid Receptor 1 (CNR1) in E. coli for Drug Screening Applications"*
**作者**: Patel S, et al.
**摘要**: 通过大肠杆菌表达系统实现CNR1重组蛋白的高效可溶性表达，开发了一种快速纯化方法，并验证其在基于细胞膜的药物筛选平台中的应用潜力。
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这些研究涵盖了CNR1重组蛋白在不同表达系统(酵母、昆虫细胞、大肠杆菌)中的表达优化、纯化策略及其在结构解析或药物开发中的应用。
The cannabinoid receptor type 1 (CNR1), also known as CB1. is a G protein-coupled receptor (GPCR) predominantly expressed in the central nervous system (CNS), with roles in regulating neurotransmission, pain perception, appetite, and mood. As a key component of the endocannabinoid system, CNR1 interacts with endogenous ligands like anandamide and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids (e.g., THC) and synthetic compounds. Recombinant CNR1 protein is engineered for in vitro studies to elucidate its structure, signaling mechanisms, and therapeutic potential. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the recombinant protein retains functional domains, including seven transmembrane helices and ligand-binding pockets, often fused with tags (e.g., His-tag) for purification and detection. Its applications include drug screening for agonists/antagonists targeting metabolic disorders, neurodegenerative diseases, and pain management, as well as structural studies using cryo-EM or X-ray crystallography. Research on recombinant CNR1 has advanced understanding of GPCR signaling and contributed to developing cannabinoid-based therapies with reduced psychoactive side effects. However, challenges remain in maintaining receptor stability and native conformation in vitro. Ongoing efforts focus on optimizing expression systems and biophysical assays to improve drug discovery workflows.
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