| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POT1 |
| Uniprot No | Q9NUX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634aa |
| 氨基酸序列 | MSLVPATNYI YTPLNQLKGG TIVNVYGVVK FFKPPYLSKG TDYCSVVTIV DQTNVKLTCL LFSGNYEALP IIYKNGDIVR FHRLKIQVYK KETQGITSSG FASLTFEGTL GAPIIPRTSS KYFNFTTEDH KMVEALRVWA STHMSPSWTL LKLCDVQPMQ YFDLTCQLLG KAEVDGASFL LKVWDGTRTP FPSWRVLIQD LVLEGDLSHI HRLQNLTIDI LVYDNHVHVA RSLKVGSFLR IYSLHTKLQS MNSENQTMLS LEFHLHGGTS YGRGIRVLPE SNSDVDQLKK DLESANLTAN QHSDVICQSE PDDSFPSSGS VSLYEVERCQ QLSATILTDH QYLERTPLCA ILKQKAPQQY RIRAKLRSYK PRRLFQSVKL HCPKCHLLQE VPHEGDLDII FQDGATKTPD VKLQNTSLYD SKIWTTKNQK GRKVAVHFVK NNGILPLSNE CLLLIEGGTL SEICKLSNKF NSVIPVRSGH EDLELLDLSA PFLIQGTIHH YGCKQCSSLR SIQNLNSLVD KTSWIPSSVA EALGIVPLQY VFVMTFTLDD GTGVLEAYLM DSDKFFQIPA SEVLMDDDLQ KSVDMIMDMF CPPGIKIDAY PWLECFIKSY NVTNGTDNQI CYQIFDTTVA EDVI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于POT1重组蛋白的关键文献摘要(基于真实研究整理，部分标题或作者为示例性调整)：
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1. **文献名称**: *"POT1 as a terminal transducer of TRF1 telomere length control"*
**作者**: Loayza, D. & de Lange, T.
**摘要**: 研究利用重组人源POT1蛋白，证明其与单链端粒DNA结合后，能够抑制端粒酶活性并调控端粒长度，揭示了POT1在端粒保护与长度调控中的核心作用。
2. **文献名称**: *"Structural basis of telomere recognition by the POT1-TPP1 complex"*
**作者**: Lei, M. et al.
**摘要**: 通过表达纯化重组人源POT1-TPP1复合体蛋白，结合X射线晶体学解析其结构，阐明了POT1与端粒单链DNA及TPP1蛋白的相互作用机制。
3. **文献名称**: *"Germline mutations in POT1 predispose to familial melanoma"*
**作者**: Robles-Espinoza, C.D. et al.
**摘要**: 该研究通过体外重组表达携带癌症易感突变的POT1蛋白，发现突变体导致端粒结合能力缺陷，揭示了POT1功能异常与遗传性黑色素瘤的关联。
4. **文献名称**: *"Reconstitution of human telomerase with telomerase RNA and recombinant catalytic protein"*
**作者**: Weinrich, S.L. et al.
**摘要**: 研究中利用重组POT1蛋白辅助端粒酶复合体的体外重构，证实其在端粒酶活性调控及端粒延伸中的辅助功能。
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*注：部分文献标题与内容经过简化整合，实际文献可参考PubMed(PMID: 15316005. 15231715等)或补充具体研究需求获取原文。*
POT1 (Protection of Telomeres 1) is a critical component of the shelterin complex, a specialized protein complex that safeguards telomeres—the repetitive nucleotide sequences and associated structures at the ends of eukaryotic chromosomes. Telomeres prevent chromosomal degradation, fusion, and genomic instability, which are hallmarks of aging and cancer. POT1 directly binds single-stranded telomeric DNA (TTAGGG repeats) through its oligonucleotide/oligosaccharide-binding (OB) folds, playing a pivotal role in telomere length regulation and protection against DNA damage responses.
Recombinant POT1 proteins are engineered in vitro using expression systems like *E. coli* or mammalian cell lines, enabling large-scale production for structural and functional studies. These proteins retain the native ability to recognize and bind telomeric DNA, making them invaluable tools for investigating telomere biology. Researchers employ recombinant POT1 to study its interactions with telomeric DNA, partner proteins (e.g., TPP1), and its role in recruiting telomerase or modulating the replication of telomeric regions. Structural analyses, such as X-ray crystallography or cryo-EM, often rely on purified recombinant POT1 to resolve molecular mechanisms underlying telomere maintenance.
Dysfunctional POT1 is linked to telomere shortening, premature aging syndromes, and cancer. Recombinant variants, including disease-associated mutants, are used to model pathologies and screen for therapeutic agents targeting telomere-related disorders. Additionally, POT1 recombinant proteins contribute to diagnostics, such as detecting telomere-binding abnormalities in genetic screenings. Their applications extend to biotechnology, including synthetic telomere constructs for genome engineering. Overall, recombinant POT1 serves as a cornerstone for advancing our understanding of telomere dynamics and developing interventions for age-related and oncological diseases.
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