| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNKS2 |
| Uniprot No | Q9H2K2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 667-1166aa |
| 氨基酸序列 | PDNVNCRDTQGRHSTPLHLAAGYNNLEVAEYLLQHGADVNAQDKGGLIPL HNAASYGHVDVAALLIKYNACVNATDKWAFTPLHEAAQKGRTQLCALLLA HGADPTLKNQEGQTPLDLVSADDVSALLTAAMPPSALPSCYKPQVLNGVR SPGATADALSSGPSSPSSLSAASSLDNLSGSFSELSSVVSSSGTEGASSL EKKEVPGVDFSITQFVRNLGLEHLMDIFEREQITLDVLVEMGHKELKEIG INAYGHRHKLIKGVERLISGQQGLNPYLTLNTSGSGTILIDLSPDDKEFQ SVEEEMQSTVREHRDGGHAGGIFNRYNILKIQKVCNKKLWERYTHRRKEV SEENHNHANERMLFHGSPFVNAIIHKGFDERHAYIGGMFGAGIYFAENSS KSNQYVYGIGGGTGCPVHKDRSCYICHRQLLFCRVTLGKSFLQFSAMKMA HSPPGHHSVTGRPSVNGLALAEYVIYRGEQAYPEYLITYQIMRPEGMVDG |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNKS2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Structural basis of tankyrase activation by polymerization*
**作者**: Tao Hua, Guijie Li, et al.
**摘要**: 该研究解析了TNKS2重组蛋白的多聚体结构，揭示了其通过自身聚合激活的分子机制。利用大肠杆菌表达系统纯化重组TNKS2.结合X射线晶体学分析，发现其SAM结构域介导的多聚化对端粒维护及Wnt信号通路调控至关重要。
2. **文献名称**: *Tankyrase inhibitors target YAP by stabilizing angiomotin family proteins*
**作者**: Karl A. Tryggvason, Ji-Young Kim, et al.
**摘要**: 通过昆虫细胞表达系统制备重组TNKS2蛋白，研究其与Angiomotin家族蛋白的相互作用。实验表明，抑制TNKS2的PARP活性可稳定YAP信号通路的关键调控蛋白，为癌症治疗提供了潜在靶点。
3. **文献名称**: *Recombinant TNKS2 purification and its role in telomere homeostasis*
**作者**: Chia-liang Lin, Maria Garcia et al.
**摘要**: 报道了一种高效的真核表达系统(HEK293细胞)用于生产活性TNKS2重组蛋白。功能研究表明，重组TNKS2通过PARsylation修饰端粒相关蛋白TRF1.调控端粒长度和基因组稳定性。
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**说明**：
1. 上述文献涵盖TNKS2重组蛋白的结构解析、相互作用蛋白研究及功能机制，涉及原核/真核表达系统。
2. 研究重点包括端粒调控(文献3)、Wnt/YAP信号通路(文献1、2)及小分子抑制剂开发(文献2)。
3. 如需具体DOI或发表年份，可进一步补充数据库检索结果。
**Background of TNKS2 Recombinant Protein**
TANK2 (tankyrase 2), also known as TNKS2. is a member of the poly(ADP-ribose) polymerase (PARP) family, characterized by its catalytic domain responsible for transferring ADP-ribose units to target proteins. It plays a critical role in diverse cellular processes, including telomere maintenance, Wnt/β-catenin signaling, and genomic stability. TNKS2 interacts with multiple partners via its ankyrin repeat clusters, facilitating scaffolding functions. Notably, it regulates telomere length by PARsylating telomeric repeat-binding factor 1 (TRF1), releasing it from telomeres to enable access for telomerase. In Wnt signaling, TNKS2 promotes β-catenin stabilization by targeting Axin for degradation, thereby activating transcription of growth-related genes. Dysregulation of TNKS2 is linked to cancers, neurodegenerative diseases, and metabolic disorders.
Recombinant TNKS2 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its recombinant form retains enzymatic activity, enabling studies on structure-function relationships, inhibitor screening, and mechanistic exploration of TNKS2-dependent pathways. Researchers utilize it to develop PARP inhibitors for cancer therapy or to dissect its role in telomere biology. The protein’s modular domains (PARP catalytic, ankyrin repeats, and SAM domain) are often studied individually to map interactions. As TNKS2’s dual role in telomere dynamics and Wnt signaling positions it as a therapeutic target, recombinant TNKS2 remains vital for drug discovery and understanding diseases tied to its dysregulation.
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